Adipose tissue in overweight individuals with and without the metabolic syndrome; differences in free fatty acid profile in Fasting and Fed state using 1H-MR-spectroscopy.
Completed
- Conditions
- adipose tissue dysfunctionoverweight1001842410003216
- Registration Number
- NL-OMON39951
- Lead Sponsor
- niversitair Medisch Centrum Utrecht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
1. Between ages of 18 and 70 years.
2. Male or female
3.a. Metabolic syndrome group: BMI *25 kg/m2 and presence of the metabolic syndrome.
3.b. No metabolic syndrome group: BMI *25 kg/m2 and absence of the metabolic syndrome.
Exclusion Criteria
1. Known cardiovascular disease
2. Known renal, liver or pulmonary disease
3. Diabetes mellitus
4. Use of medication (except for oral anticonceptives, proton pump inhibitor*s, inhalation medication or topical unguents)
5. Pregnancy or lactation
6. Claustrophobia
7. Waist circumference too large to fit MRI (>200cm)
8. Metallic devices in the body of the participant
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>1. Free fatty acid ratios<br /><br>Triglycerides (TG), poly unsaturated fatty acids (PUFA) and total unsaturated<br /><br>fatty acids (TUFA) will be measured with spectroscopy.<br /><br>The PUFA/TG, TUFA/TG and the PUFA/TUFA ratio will be calculated after<br /><br>standardization of the measurements with respect to voxelsize and watercontent.<br /><br><br /><br>2. Serum triglycerides vs adipose tissue triglycerides<br /><br>The change in concentration of triglycerides in serum (peripheral blood) will<br /><br>be compared to the change in concentration of triglycerides in the adipose<br /><br>tissue measured with spectroscopy, both before and after an oral fat load. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Not applicable </p><br>