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Vaginal CO2 Laser and the Genitourinary Syndrome of Menopause

Not Applicable
Conditions
Vaginal Atrophy
Interventions
Device: Microablative Fractional CO2 laser
Registration Number
NCT03754205
Lead Sponsor
National and Kapodistrian University of Athens
Brief Summary

This study evaluates the clinical, histopathological, cytological and microbiological efficacy of Microablative Fractional CO2 laser intravaginally administered in postmenopausal women with Genitourinary Syndrome of Menopause. Half participants will receive active CO2 laser therapy, while the other half will receive placebo CO2 laser therapy.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
Female
Target Recruitment
60
Inclusion Criteria
  • Symptoms of vaginal atrophy (dyspareunia and/or vaginal dryness)
  • Menopause
Exclusion Criteria
  • Systemic or local hormonal therapy the last 6 months
  • Use of moisturizers or lubricants the last month
  • Active genital infection (i.e herpes, vaginitis)
  • Prolapse stage >=2
  • Underlying pathologies that could interfere with the protocol compliance

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Placebo GroupMicroablative Fractional CO2 laserPlacebo CO2 laser therapies at monthly intervals. The laser parameters that will be used are the following: 1) Power: 0.5 watts, 2) Dwell time:1000μs, 3) Spacing 1000 μm, 4) Depth: SmartStak parameter 1, 5) Smart-pulse mode.
Laser GroupMicroablative Fractional CO2 laserMicroablative Fractional CO2 laser therapy at monthly intervals. The laser parameters that will be used are the following: (1) Power: 30 ή 40 watts, 2) Dwell time:1000μs, 3) Spacing 1000 μm, 4) Depth: SmartStak parameter from 1-3 depending on the treatment status, 5) D-pulse mode.
Primary Outcome Measures
NameTimeMethod
10-centimeter Visual Analogue Scale assessing intensity of dyspareunia and dryness24-months

It is a straight line with 2 endpoints "0" and "10". Zero defines "no dyspareunia at all" and "no dryness at all" , while 10 "dyspareunia as bad as it could be" and "dryness as bad as it could be". Participants will draw a line at or between the 2 end points. The distance between zero and drawn line will define the intensity of dyspareunia and dryness

Secondary Outcome Measures
NameTimeMethod
Polymerase Chain Reaction24 months

Lactobacilli, bacteria and fungi will be evaluated.

Density of blood vessels24 months

Computerized determination of volume density of blood vessels in percent of total area of subepithelial stroma will be performed

Day-to Day Impact of vaginal aging questionnaire (DIVA)24 months

It includes 4 domains: activities of daily living (5 items), sexual functioning (5 items), emotional well-being (4 items), self-concept and body image (5-items). Each item of each domain could receive values from 0 to 4. Mean scores are calculated on each of the domain scales. Higher scores indicate greater symptom impact

King's Health Questionnaire (KHQ)24 months

It has 3 sections: 1) general health and overall health related to urinary symptoms with 2 questions, 2) incontinence impact, role limitations, physical limitations, social limitations, personal limitations, emotions, sleep and energy, and severity coping measures with 19 questions and 3) bother or impact of urinary symptoms with 11 questions. Scores of each domain of the 2 sections range from 0 to 100. Scores of the third section range from 0 to 3. Higher scores indicate higher impact of urinary incontinence.

Patients Global Impression of Improvement24 months

It is a single question with 7 possible answers. Patients will chose the answer that applies at their impression of improvement after the laser therapy

3 days voiding diary24 months

Assesses bladder function (frequency, urgency and incontinence)

Number of Blood vessels24 months

Computerized determination of number of capillary profiles in the test area will be performed percent of total area of subepithelial stroma), will be performed.

Size of blood vessels24 months

Computerized determination of diameter (in μ), length (in μ) αnd width (in μ) will be performed.

Female Sexual Function Index24 months

It includes 6 domains assessing sexual functioning (desire, arousal, orgasm, lubrication, satisfaction and pain). Total score is calculated by summing scores of the 5 domains multiplied by certain factors with a minimum value of 2 and maximum 36

Vaginal Health Index Score24 months

It evaluates vaginal elasticity, fluid volume, ph of vaginal fluid, epithelial integrity and moisture. Each one of these may receive scores from 1 to 5. Total score is calculated by summing the 5 scores ranging from 5 to 25. Higher scores indicate better vaginal status.

Cultures of vaginal samples24 months

Lactobacilli, aerobic bacteria, anaerobic bacteria, fungi will be evaluated

10-centimeter Visual Analogue Scale assessing intensity of itching, burning and dysuria24 months

It is a straight line with 2 endpoints "0" and "10". Zero defines "no symptom at all", while 10 "symptom as bad as it could be" and "dryness as bad as it could be". Participants will draw a line at or between the 2 end points. The distance between zero and drawn line will define the intensity of symptom

International Consultation on Incontinence Questionnaire Short Form/Female Lower Urinary tract Symptoms (ICIQ-FLUTS)24 months

It is a 12-items questionnaire evaluating nocturia, urgency, bladder pain, frequency, hesitancy, straining, intermittency, urinary incontinence (urge, stress and unexplained), frequency of urinary incontinence and nocturnal enuresis. Filling, voiding and incontinence symptoms subscales range from 0 to 15, 0 to 12 and 0 to 20, respectively.

Vaginal Maturation Value24 months

It is calculated by defining the percentage of superficial, intermediate and parabasal epithelial cells on the vaginal pap smear, following the formula (1x%superficial)+(0.5x%intermediate)+(0x%parabasal). It may receive values from 0 to 100%.It is considered to be an indicator of the estrogenic stimulation, whereas values of 0-49%, 50-64% and 65-100% indicate absent/low, moderate and high estrogenic effect on the vaginal epithelium, respectively.

Epithelial thickness24 months

Biopsies will be obtained from the lateral vaginal wall. Epithelial thickness (in μ) will be measured by a vertical line between basal layer cells and superficial layers.

Trial Locations

Locations (1)

Urogynecological Unit of Alexandra Hospital

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Athens, Greece

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