Endothelial Colony Forming Cells, Endothelial Progenitor Cells and Bicuspid Aortic Valves: in vitro culture of peripheral blood cells to study biomarkers and pathogenesis in BAV?

Recruiting

• Conditions: Bicuspid aortic valves; two aortic valves; 10046973; 10007510; 10002363

• Registration Number: NL-OMON46125
• Lead Sponsor: Anatomie

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 23 April 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 400

Inclusion Criteria

• aged >= 18 years;
• capable of understanding and signing informed consent.
• echocardiogram at most 1 year and 2 months before blood drawing
• for the patient population: Bicuspid aortic valves

Exclusion Criteria

• Consuming more than 3 units of alcohol the evening before drawing blood
For the control population:
• Valvular stenosis
• Regurgitation
• Aortic dilation

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The parameters studied are endothelial cell communication and endothelial cell<br /><br>function. -<br /><br>- The cell communication will be primarily studied in the ECFCs. The response<br /><br>of smooth muscle cells to the presence of ECFC secreted factors will determine<br /><br>what the focus will be of the study. There are 4 likely candidates:<br /><br>inflammatory factors, growth factors, nitric oxide regulation and extracellular<br /><br>matrix remodelling.<br /><br>- Endothelial cell function will be studied in the EMT response, migration and<br /><br>calcification.<br /><br>- The results from the ECFCs will then be studied in the EPC*s to look for<br /><br>potential biomarkers</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>The results obtained with the ECFC*s will studied for potential links to<br /><br>valvular development. This might elucidate the pathogenesis of bicuspid aortic<br /><br>valves.</p><br>