Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 59
- 试验地点
- 2
- 主要终点
- The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic
研究概览
简要总结
Introduction: Recently it has involved the role of TLR (toll-like receptors) in the pathogenesis of asthma. TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters (PAMPs). The possible role that TLRs play in IgE-dependent asthma (not eosinophilic) and its recognition as a new endotype asthma, could open new therapeutic expectations.
Objective: To analyze the expression of TLR 2 and 4 in monocytes/ macrophages and neutrophils in peripheral blood and induced sputum from asthmatic patients
详细描述
The investigators studied patients with asthma. All patients underwent the same day an induced sputum, pulmonary function studies, fractional exhaled nitric oxide, blood total IgE and skin test by prick technique. Asthmatic patients were classified according to the criteria of the GINA 2012. The cellular composition was analyzed by flow cytometry. Expression of TLR2 and TLR4 were analyzed using monoclonal antibodies conjugated with fluorochromes appropriate.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adults with persistent asthma (defined as per the Global INitiative for Asthma Management (GINA) criteria)
排除标准
- •If they had a respiratory tract infection or asthma exacerbation within 30 days prior to inclusion
- •If they were receiving oral steroids or immunosuppressive treatments.
研究组 & 干预措施
toll like receptor (TLRs)
TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters
干预措施: TLRs (Other)
结局指标
主要结局
The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic
时间窗: 2 years
The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic (IgEnodep).This could suggest that in this type of asthma the defense mechanism of neutrophils could be altered by decreasing expression of these receptors on their cell surface. This mechanism would still be defined. Probably the respiratory infections play an important role.
次要结局
未报告次要终点
研究者
Astrid Crespo
Department of Respiratory Medicine, Hospital de la Santa Creu i Sant Pau. Institut d'Investigació Biomédica Sant Pau (IIB Sant Pau). Universitat Autònoma de Barcelona, Department of Medicine. Barcelona Respiratory Network (BRN). Barcelona, Spain.
Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau