Diet-induced alteration of microbiota and development of obesity, nonalcoholic fatty liver disease and diabetes

Completed

• Conditions: diabetes; insulin resistance; 10018424

• Registration Number: NL-OMON47711
• Lead Sponsor: Maxima Medisch Centrum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 11 June 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 228

Inclusion Criteria

BMI > 40 kg/m2 or > 35 kg/m2 with comorbidities
Undergoing a laparoscopic gastric bypass
Age between 18 and 65;Control population:
BMI between 20-25 kg/m2
Indication for laparoscopic cholecystectomy
Indication for gastroscopy
Age 18 to 65 years

Exclusion Criteria

Both groups:
Type 1 diabetes
Alcohol abuse or drug abuse
Inflammatory illness, such as auto-immune disease
Degenerative disease
Physician prescribed use of corticosteroids and prednisone;Controlgroup
Diabetes type 2
Cachexia

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>* Bodyweight, BMI, abdominal circumference, plasma glucose/insulin,<br /><br>lipidspectrum<br /><br>* Faecal and intestinal microbiota composition<br /><br>* Plasma en faecal Short Chain Fatty Acids, Glucagon-like-peptide-1<br /><br>* Paneth cel typing by determination of luminal en faecal concentrations of<br /><br>lysozym/HD5/HD6/LL-37/sPLA2/HIP/PAP<br /><br>* Intestinal protein en mRNA expression and localisation of lysozym/HD5/HD6<br /><br>* Mucus typing by determination of the number of goblet cells, mucin 2 protein<br /><br>concentration en glycosylation.<br /><br>* Volatile Organic Compounds (VOC) in exhailed air<br /><br>* Fecal calprotectin in obese subjects</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>nvt</p><br>