Negative Pressure Wound Therapy and Allogeneic Human Skin Grafts for Wound Bed Preparation

Not Applicable

Withdrawn

• Conditions: Necrotizing Soft Tissue Infection
• Interventions: Procedure: Negative pressure wound therapy; Procedure: Glycerol Preserved Allografts (GPA)

• Registration Number: NCT02314468
• Lead Sponsor: University Hospital, Ghent

Brief Summary

Introduction Necrotising soft tissue infections (NSTI) incorporate a spectrum of pathologies, all characterized by an infectious state, typically arising after a penetrating trauma or a surgical procedure and an expeditious spreading of necrosis throughout the soft tissues of the body. It is a rare, life-threatening and devastating infection defined by a necrosis of fascia, subcutaneous tissues and skin. Aggressive surgical debridement to remove all necrotic tissue and define the extent of the disease is still the mainstay of correct treatment of NSTI.

Both negative pressure wound therapy (NPWT) and the application of allograft skin to debrided areas, are documented options for wound bed preparation which are standard in the university hospital of Gent.

NPWT is a technique for wound bed preparation involving the controlled application of sub-atmospheric pressure to the local wound environment, using a sealed wound dressing connected to a vacuum pump. Mechanisms of action attributed to NPWT include an increase in blood flow, promotion of angiogenesis, reduction in wound surface area, positive modulation of the inhibitory contents of wound fluid, induction of cell proliferation, reduction of edema, and bacterial clearance.

Allograft skin or cadaveric skin possesses many of the ideal properties of biologic dressings, and plays a major role in the surgical management of extensive wounds when autologous tissue may not be immediately available. It reduces evaporative water loss and the drainage of protein-rich fluids, prevents wound desiccation, and suppresses microbial proliferation. Wound pain is lessened and the allograft restores a physiologic barrier at the wound surface. Enhancing revascularization, and thereby creating a viable wound bed before final reconstruction, is perceived as one of the most important features of allografting.

Detailed Description

Study objectives This study will compare negative pressure wound therapy versus cadaveric skin as treatment options for wound bed preparation in wounds resulting from necrotising soft tissue infection.

Methodology One arm includes a NPWT system that is used in conjunction with gauze or foam dressings. Dressing changes normally will be carried out twice a week unless otherwise indicated by the wound condition, the patients clinical presentation or a seal broken beyond repair. NPWT wound bed preparation will be ended when two experienced plastic surgeons consider the wound bed suitable for autografting. (Endpoint) The second arm includes application of cadaveric skin. The allografts normally will be changed every seven to ten days or earlier depending on adhesion of the allograft to the wound bed. In practice, the ability of the allograft to adhere to the wound bed has a diagnostic value, referred to as a 'take-test'. If the allograft does not adhere, one must consider an infection or non-viable wound surface. If the allograft adheres to the wound bed and adequate granulation tissue is suspected underneath, then the wound bed is suitable for autografting. (Endpoint)

Study Timeline

• Study First Submitted: 2013-09-30
• Study Start: 2013-10
• Study First Submitted QC: 2014-12-08
• Study First Posted: 2014-12-11
• Primary Completion: 2016-06
• Study Completion: 2016-10
• Status Verified: 2023-02
• Last Update Submitted: 2023-02-07
• Last Update Posted: 2023-02-10

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: All
• Target Recruitment: Not specified

Inclusion Criteria

• Necrotising soft tissue infection

Exclusion Criteria

None specific

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
negative pressure wound therapy (NPWT)	Negative pressure wound therapy	negative pressure wound therapy (NPWT)
Glycerol Preserved Allografts (GPA)	Glycerol Preserved Allografts (GPA)	Glycerol Preserved Allografts (GPA)

Primary Outcome Measures

Name	Time	Method
Quality of wound bed preparation until autografting.	After 3 days	WHAT software, clinical assessment by plastic surgeon, Laser Doppler Imaging scan.

Secondary Outcome Measures

Name	Time	Method
Cost utility until autografting.	After 3 days	Cost of material.
Pain assessment until autografting.	After 3 days	Numerical pain scale.
Ease of use until autografting.	After 3 days	Numerical scale for ease of use.

Trial Locations

Locations (1)

University Hospital Ghent; 🇧🇪 Ghent, Belgium