Single Cell Leukocyte Landscapes and Cardiovascular Risk in Children With Chronic Kidney Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 38
- 试验地点
- 1
- 主要终点
- Immunephenotyping of human PBMC
研究概览
简要总结
Chronic kidney disease (CKD) is associated with an increased cardiovascular mortality. In particular children with early-onset CKD have a lifelong increased risk to suffer from cardiovascular disease (CVD). Therefore, children with CKD deserve our attention. The immune system in children with CKD is disturbed, exhibiting pro-inflammatory features. Therefore, we aim to learn more about the characteristics of the immune system in early-onset CKD. In this project PBMC of pediatric CKD patients and age-matched healthy controls will be analysed and compared using CITE-Seq as a multimodal scRNAseq phenotyping method. All patients will be clinically characterized to integrate cardiovascular and immunological data.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 5 Years 至 18 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •matching to one of the following groups CKD G3-5: estimated eGFR according to Schwartz formula <60ml/min*1,73m2 (no ESKD) CKD G5 D: patients with ESKD treated with hemodialysis for at least 3 months normal kidney function: CKD G1 or no CKD with eGFR > 90ml/min*1,73m2
- •informed consent to participate in this study
排除标准
- •body weight of less than 15kg
- •acute or chronic inflammatory diseases
- •chronic liver disease
- •inflammatory bowel disease or other gastrointestinal disorders (constipation, diarrhea, short bowel syndrome)
- •antibiotic prophylaxis or treatment within four weeks prior to recruitment
结局指标
主要结局
Immunephenotyping of human PBMC
时间窗: at recruitment
scRNAseq with Cellular Indexing of Transcriptomes and Epitopes (CITE)-Seq for whole PBMC will be used to gain information on single cell transcriptomes across multiple immune cell populations together with expression levels of classical mmunological surface markers.
次要结局
- Pulse wave velocity(at recruitment)
- Echocardiography(at recruitment)
- Targeted metabolomics of plasma samples(at recruitment)
- Carotid intima media thickness(at recruitment)
- Microbiome sequencing of fecal samples(at recruitment)
研究者
Johannes Benjamin Holle
Dr. med.
Charite University, Berlin, Germany