Methionine and Cysteine Restriction in Humans
- Conditions
- Overweight
- Interventions
- Behavioral: Low met/cys dietBehavioral: High met/cys dietBehavioral: Moderate met/cys diet
- Registration Number
- NCT03629392
- Lead Sponsor
- University of Oslo
- Brief Summary
The aim of this study is to examine the effect of methionine and cysteine restriction on energy and macronutrient metabolism in overweight human subjects
- Detailed Description
The sulfur-containing amino acid methionine and its downstream metabolite cysteine has been associated with fat mass in animal studies and human observational studies. Some of the proposed mechanisms include an association with lipogenesis, insulin sensitivity and disrupted energy metabolism.
The overall aim of this project is to evaluate the effects of a diet low in methionine and cysteine on markers of lipids, amino acids, glucose and other biomarkers related to energy metabolism, including transcriptomic and protein expression data from adipose tissue and lymphocytes. Overweight and obese but otherwise healthy volunteers will be randomized to receive a diet either low, moderate or high in methionine and cysteine concentrations for 1 week.
This is a pilot study that may be considered an extension of NCT02647970. In addition to the primary and secondary outcomes we will evaluate the feasibility of the intervention and procedures.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 19
- Body mass index of 25-35 kg/m2
- High physical activity
- Smoking
- Drugs
- Chronic disease
- Pregnancy or breastfeeding
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Low met/cys diet Low met/cys diet Dietary intervention High met/cys diet High met/cys diet Dietary intervention Moderate met/cys diet Moderate met/cys diet Dietary intervention
- Primary Outcome Measures
Name Time Method Change in plasma concentrations of amino acids Baseline, Day 1, Day 3 and Day 7 (final visit) Measurement of biomarkers of amino acid metabolism, including plasma concentrations of methionine, cysteine and related metabolites
Change in protein expression activity in tissues Baseline and day 7 (final visit for adipose tissue, Baseline and day 1, 3 and 7 (final visit) for lymphocytes. Measurement of protein expression and activity related to methionine and cysteine metabolism in adipose tissue and lymphocytes.
- Secondary Outcome Measures
Name Time Method Change in plasma activity indices of steaoryl-CoA desaturase Baseline, Day 1, Day 3 and Day 7 (final visit) Calculated based onthe ratio of plasma concentrations of products and precursors
Change in protein expression of steaoryl CoA-desaturase in adipose tissue Baseline and Day 7 (final visit) Measurement of protein expression of steaoryl CoA-desaturase in adipose tissue
Change in plasma concentrations of fatty acids Baseline, Day 1, Day 3 and Day 7 (final visit) Measurement of fatty acid content in plasma
Change in protein expression of steaoryl CoA-desaturase in lymphocytes Baseline, Day 1, Day 3 and Day 7 (final visit) Measurement of protein expression of steaoryl CoA-desaturase in lymphocytes
Change in steaoryl CoA-desaturase activity in adipose tissue Baseline and Day 7 (final visit) Measurement of enzyme activity of steaoryl CoA-desaturase in adipose tissue
Diet feasibility Baseline, Day 1, Day 3 and Day 7 (final visit) Diet feasibility will be measured by a self-administered questionnaire
Diet compliance Baseline, Day 1, Day 3 and Day 7 (final visit) Compliance with the diet will be measured by a self-administered questionnaire
Body composition Baseline and day 7 (final visit) Measurement of body composition by bioimpedance and dual X-ray absorptometry
Trial Locations
- Locations (1)
University of Oslo
🇳🇴Oslo, Norway