Lipids and Other Bioactive Compounds of Human Breast Milk and Infant Formulas.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 7
- 试验地点
- 2
- 主要终点
- Relative proportions (%) of fatty acids in triacylglycerols of human milk.
研究概览
简要总结
The biochemical differences in the composition of breast milk and formula have not been profoundly established in all compound groups. Increased understanding of breast milk composition, the normal variation of various bioactive compounds and their levels is required to be able to develop formulas better corresponding the breast milk, and ensure the optimal development of also the formula-fed babies.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Other
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •breast-fed child was born full-time and is healthy.
- •age of the breast-fed child is 0-6 months
排除标准
- •breast-fed child was born prematurely (gestation week <37)
结局指标
主要结局
Relative proportions (%) of fatty acids in triacylglycerols of human milk.
时间窗: 11/2018-12/2019
Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Quantitation with gas chromatograph coupled with flame ionization detector in relation to external standards.
Relative proportions (%) of regioisomers within selected molecular species (acyl carbon number:number of double bonds 50:1 and 52:2) in triacylglycerols.
时间窗: 11/2018-12/2019
Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Negative ionization of the triacylglycerols with ammonia. Selection of the molecular weight species in the first quadruple of the MS/MS. Fragmentation of the selected ions with argon. Separation of the fragment ions in the second quadrupole of the MS/MS. Calculation of the relative proportions of regioisomers with a computer software.
Quantity (mg/ml) of lipid soluble compounds in triacylglycerols of human milk.
时间窗: 11/2018-12/2019
Isolation of lipids from the milk with solvent extraction. Gravimetric analysis of the lipids.
次要结局
未报告次要终点
研究者
Kaisa Linderborg
Associate Professor
University of Turku