A comparison of two modalities to visualise the microcirculation: Contrast Enhanced Ultrasonography and capillary videomicroscopy

Recruiting

Conditions: disturbed vasodilation
Microvascular dysfunction
10018424
10057166

Registration Number: NL-OMON33075

Lead Sponsor: Vrije Universiteit Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: Not specified

Target Recruitment: 20

Inclusion Criteria

1. Caucasian*
2. age 18-55 years
3. 20 < BMI > 35
* because capillaries are not visible in the nailfold in a pigmented skin

Exclusion Criteria

1. cardiovascular disease (stroke, coronary artery disease, peripheral vascular disease, heart failure)
2. pulmonary disease
3. diabetes mellitus (FPG > 7.8 mmol/l)
4. liver dysfunction (ASAT, ALAT and/or alkaline phosphatase >3 times the upper limit of normal)
5. renal failure
6. smoking
7. alcohol use > 4 U/day
8. use of medication (antihypertensive drugs, lipid lowering drugs, corticosteroids, NSAIDs, ciclosporin A, rifampicin)
9. pregnancy
10. insufficient knowledge of the Dutch language.
11. known previous allergic reaction to ultrasound contrast-agent (e.g. Sonovue)

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. percentage change in muscle microvascular blood volume as measured by CEUS.<br /><br>2. percentage change in number of capillaries as measured by nailfold<br /><br>capillaroscopy.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1. muscle microvascular flow velocity (percentage change)<br /><br>2. muscle microvascular blood flow (percentage change)<br /><br>3. time to peak flow<br /><br>4. muscle microvascular flow distribution<br /><br>5. whole body glucose uptake<br /><br>6. total forearm blood flow (percentage change)<br /><br>7. blood pressure</p><br>