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Intestinal Microbiota Composition in Patients With Chronic Pancreatitis and Pancreatic Exocrine Insufficiency

Completed
Conditions
Chronic Pancreatitis
Pancreatic Exocrine Insufficiency
Interventions
Combination Product: enzyme replacement therapy
Registration Number
NCT05132309
Lead Sponsor
Federal Research and Clinical Center of Physical-Chemical Medicine
Brief Summary

Assessment of qualitative and quantitative composition of the intestinal microbiota in patients with CP; in patients with CP and PEI complication of mild to severe degree; patients with CP and PEI who underwent surgical intervention on the pancreas. Intergroup comparison of the microbial compositional characteristics and their analysis. Correction of enzyme replacement therapy in selected groups of patients with regard to the identified microbial characteristics. Assessment of the intestinal microbiota composition in dynamics after 6 (+1) months on the background of allocated therapy

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
96
Inclusion Criteria
  • Age 18 to 80 years old; any gender;
  • Chronic pancreatitis etiology: idiopathic, biliary dependent, drug-induced, alcoholic, dysmetabolic, CP as a consequence of recurrent and severe acute pancreatitis (postnecrotic);
  • History of confirming diagnostic criteria of CP (in accordance with 1) Clinical guidelines of the Russian Gastroenterological Association on the diagnosis and treatment of exocrine pancreatic insufficiency 2018; 2) Clinical guidelines of the unified European guidelines for gastroenterology, on the diagnosis and therapy of chronic pancreatitis 2017; 3) Clinical guidelines of the American College of Gastroenterology 2020);
  • CP diagnosis according to multispiral computed tomography of the abdominal cavity and/or endoscopic ultrasonography of pancreas: size changes; dilatation of the main pancreatic duct more than 2 mm; increased echogenicity of the walls, uneven contours and heterogeneity of the BP parenchyma, concrements or calcification of the parenchyma;
  • Clinical manifestations of exocrine insufficiency of pancreas: stool disorders (mushy/liquid stool, number of defecation acts >3 p/day, undigested food remains), steatorrhea, abdominal distention, nausea/vomiting, weight loss progression with adequate caloric intake and no other objective reasons of weight loss;
  • Fecal elastase-1 values less than 200 µg/g (for CP groups with EPI) or more than 200 µg/g (for CP group without concomitant EPI);
  • Informed patient consent to participate in the study.
Exclusion Criteria
  • Acute pancreatitis;
  • Hepatitis, cirrhosis of any etiology;
  • Any acid-dependent diseases requiring immediate or long-term proton pump inhibitor therapy (PPI): gastroesophageal reflux disease, acute gastroduodenal erosions of any etiology; Zollinger-Ellison syndrome, etc;
  • Stenosis of pylorys or duodenum;
  • Condition after gastric resection or gastrectomy;
  • Obstructive and autoimmune CP;
  • Mesenteric ischemia syndrome;
  • Any other cause of chronic diarrhea (irritable bowel syndrome with diarrhea predominance, gluten enteropathy, lactase deficiency, inflammatory bowel disease, etc.);
  • Concomitant administration of drugs directly affecting exocrine pancreatic secretion (octreotide);
  • Any concomitant diseases in the stage of decompensation (cardiovascular, renal failure, diabetes mellitus, etc.);
  • Chronic infectious diseases requiring constant (long-term) supportive therapy, including HIV infection, etc;
  • Hypersensitivity to pancreatin;
  • Use of pro-, pre-, synbiotics, week prior to inclusion in this study;
  • Use of antibiotics within 4 weeks prior to inclusion in this study;
  • Pregnancy and lactation;
  • Use of prohibited concomitant therapy during the study: pro-, pre-, synbiotics, including dietary fiber preparations and lactulose, as well as antibiotics, intestinal antiseptics, loperamide, intestinal sorbents.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Chronic pancreatitis with severe pancreatic external insufficiencyenzyme replacement therapy-
Chronic pancreatitis without pancreatic external insufficiencyenzyme replacement therapy-
Chronic pancreatitis with mild pancreatic external insufficiencyenzyme replacement therapy-
Chronic pancreatitis with severe pancreatic external insufficiency who underwent pancreatic surgerenzyme replacement therapy-
Primary Outcome Measures
NameTimeMethod
Genetic study of fecal samples of patients with CP6 months

Fecal samples of patients with CP from selected patient groups with/without PEI meet inclusion criteria will undergo 16s rRNA gene sequencing for further comparative intergroup analysis of the obtained data.

Analysis of intestinal microbiota composition of patients with CP receiving inadequate dosage of pancreatin per meal per day6 month

Analysis of intestinal microbiota composition of patients with CP from selected patient groups with/without PEI receiving inadequate dosage of enzyme replacement therapy: (less then adequate dosage) of pancreatin per meal per day

Analysis of intestinal microbiota composition of patients with CP receiving 40,000 units of pancreatin per meal per day6 months

Analysis of intestinal microbiota composition of patients with CP from selected patient groups with/without PEI receiving adequate dosage of enzyme replacement therapy: 40,000 units of pancreatin per meal per day

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (2)

Moscow State University of Medicine and Dentistry

🇷🇺

Moscow, Russian Federation

Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow (FRCC PCM)

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Moscow, Russian Federation

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