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Genotype-related Molecular Targets in the Vitamin D Pathway for Spinal Disc Diseases

Conditions
Intervertebral Disk Degeneration
Registration Number
NCT03343899
Lead Sponsor
I.R.C.C.S Ospedale Galeazzi-Sant'Ambrogio
Brief Summary

The objective of our study will be to analyze the interplay between particular VDR variants and its ligand in cells obtained from intervertebral disc and cartilage endplate and their action on cell proliferation and phenotype maintenance. The identification of novel molecular targets in the vitamin D pathway, potentially promoting pathological alterations of the tissues involved in spinal disc diseases, will allow to develop innovative and more personalized preventive and pharmacological/nutritional therapies.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
15
Inclusion Criteria

patients with spinal disc diseases

  • males and females
  • 18≤age ≤ 70 years old
  • Italian white
  • patients with spinal disc diseases that will be surgically treated

Exclusion criteria:

  • age < 18 or > 70 years old
Exclusion Criteria

Not provided

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Phenotype and genotype characterization1 year

To evaluate structural alterations of the intervertebral disc and of the cartilage endplate in patients with spinal disc diseases related to functional FokI polymorphism in the vitamin D receptor (VDR) gene.

Secondary Outcome Measures
NameTimeMethod
Disc cell functional response genotype-based2 years

To characterize cells obtained from different disc tissues (nucleus pulposus, annulus fibrosus, cartilage endplate) and to evaluate their VDR FokI genotype-related response to vitamin D treatment, regulating the cell proliferation and phenotype.

Trial Locations

Locations (1)

Alessandra Colombini

🇮🇹

Milan, Italy

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