Effect of Palm Olein Intake on Lipid Profile and Appetite Regulation

Not Applicable

Completed

Brief Summary: Aims: To investigate whether acute ingestions of palm oil will have an effect on appetite regulation

Detailed Description: Design: A randomized, single blind, cross over design including 4 single study visits separated by a week. Visit 1 is an acclimatization visit followed by visit 2,3, and 4 where different types of fat will be given.

Population: 12 healthy males and females aged between 18 and 60 years with body mass index (BMI) between 18.5- 29.9kg/m2 and normal fasting plasma glucose and no evidence of insulin resistance will be recruited. No-pre existing morbidity including cardiac, hepatic or renal disease, history of diabetes, hypertension or hyperlipidaemia.

Treatment: Acclimatization visit: At visit 1, all participants will be given a milkshake like drink containing fat, protein and carbohydrate. A cannula will be inserted for the duration of the study day but no blood will be taken. Studies show than during the first visit stress response can affect the metabolic response to the study drink resulting in inaccurate results.

Study visit 2,3,4: following the acclimatization visit, participants will attend 3 more study visits separated by a week to test the metabolic response to the following fats: palm olein, interesterified palm olein and soybean oil in random order. Participants will be asked to complete a visual analogue scale questionnaire about their appetite at each time point. Fasting and postprandial plasma glucose, insulin, appetite hormones and lipids will be measured.

Recruitment & Eligibility

Inclusion Criteria

Healthy and overweight male and female volunteers of all ethnicities (body mass index (BMI) of 18.5-29.9 kg/m2)
Age between 18-60 years (inclusive)

Exclusion Criteria

Abnormal liver function test (elevated alanine aminotransferase and aspartate transaminase)/ abnormal kidney function test (elevated plasma creatinine)
History of type 2 diabetes mellitus, cancer, stomach ulcers, drug abuse or alcoholism, gastrointestinal disorders like Crohn's disease
Smokers
On lipid/blood pressure- lowering medication/supplements
Blood pressure>140/90 mm Hg
Fasting total cholesterol > 6.2 mmol/L
Fasting triacylglyceride > 2.0 mmol/L
Subject must not be allergic to intervention
Pregnancy and breastfeeding (pregnancy test will be undertaken at the screening visit)
Subjects taking nutritional supplements or on any weight-loss programs
Subjects who gained or lost ≥ 3kg weight in the past three months
Subjects with history of hypo- and hyperthyroidism
Subjects who are anaemic and those who donated blood within three months of the study

Primary Outcome Measures

Name	Time	Method
Change From Baseline in Appetite Hormones PYY	480 minutes	blood samples will be collected at specific time points presented as minutes from consumption of mixed meal. time (minutes)=-15, -5, 15, 30, 45, 60, 90, 120, 180, 240, 300, 360, 420, 480.

Secondary Outcome Measures

Name	Time	Method
Change From Baseline in Glucose,	480 min	blood samples will be collected at specific time points presented as minutes from consumption of mixed meal. time (minutes)=-15, -5, 15, 30, 45, 60, 90, 120, 180, 240, 300, 360, 420, 480
Change From Baseline in Appetite Hormones GLP_1	ime (minutes)=-15, -5, 15, 30, 45, 60, 90, 120, 180, 240, 300, 360, 420, 480. Time Frame: 480 minutes	Change From Baseline in Appetite Hormones Hide Description: blood samples will be collected at specific time points presented as minutes from consumption of mixed meal. time (minutes)=-15, -5, 15, 30, 45, 60, 90, 120, 180, 240, 300, 360, 420, 480.

Locations (1): NIHR/Wellcome Trust Imperial Clinical Research Facility
🇬🇧
London, United Kingdom
NIHR/Wellcome Trust Imperial Clinical Research Facility
🇬🇧London, United Kingdom