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Study of the Effects of Iron Levels on the Lungs at High Altitude

Not Applicable
Completed
Conditions
Mountain Sickness
Pulmonary Hypertension
Interventions
Procedure: Venesection
Drug: Normal saline
Registration Number
NCT00952302
Lead Sponsor
University of Oxford
Brief Summary

The study hypothesis is that body iron levels are important in determining the increase in lung blood pressure that occurs in response to low oxygen levels. The purpose of this study is to determine whether this is true at high altitude, where oxygen levels are low.

Detailed Description

Pulmonary hypertensive disorders frequently complicate hypoxic lung disease and worsen patient survival. Hypoxia-induced pulmonary hypertension is also a major cause of morbidity at high altitude. Hypoxia causes pulmonary hypertension through hypoxic pulmonary vasoconstriction and vascular remodelling. These processes are thought to be regulated at least in part by the hypoxia-inducible factor (HIF) family of transcription factors, which coordinate intracellular responses to hypoxia throughout the body.

HIF is regulated through a cellular degradation process that requires iron as an obligate cofactor. In cultured cells HIF degradation is inhibited by reduction in iron (by chelation with desferrioxamine) and potentiated by iron supplementation. In humans, we have recently shown that, in laboratory experiments lasting 8 hours, acute iron supplementation blunts the pulmonary vascular response to hypoxia, while acute iron chelation with desferrioxamine enhances the response.

This suggests that iron may also affect the pulmonary artery pressure response to hypoxia over longer time periods. The purpose of this study is to investigate this link between iron and the pulmonary artery pressure response to hypoxia, through a study conducted at high altitude allowing concurrent exposure of larger numbers of participants to environmental hypoxia. We wish to explore the extent and the time-course of the effect of iron on pulmonary artery pressure. Cerro de Pascu (4,340 m) in Peru provides the unique ability to make rapid transitions from sea level to high altitude (6-8 hours by road), together with the requisite research facilities. Also, one part of this study involves recruitment of patients with chronic mountain sickness, of whom there are many living in Cerro de Pasco.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
33
Inclusion Criteria
  • sea level natives of lowland ancestry
  • generally in good health
  • detectable tricuspid regurgitation on echocardiography
Exclusion Criteria
  • any significant medical problem
  • known susceptibility to high altitude pulmonary or cerebral oedema
  • taking medications or iron supplements

CMS ARM

Inclusion Criteria:

  • diagnosis of chronic mountain sickness
  • no recent venesection therapy (within 1 year)
  • detectable tricuspid regurgitation on echocardiography

Exclusion Criteria:

  • any other significant medical problem

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
CMS - placebo firstIron sucrosePatients with chronic mountain sickness (CMS) who are venesected and studied for several weeks. In the final crossover period of the study, patients receive a placebo (saline) infusion first followed by iron infusion.
CMS - placebo firstVenesectionPatients with chronic mountain sickness (CMS) who are venesected and studied for several weeks. In the final crossover period of the study, patients receive a placebo (saline) infusion first followed by iron infusion.
SLR - placeboNormal salineSea level residents (SLR) taken to high altitude for one week, and receiving placebo (saline) infusion on Day 3 at high altitude.
CMS - ironVenesectionPatients with chronic mountain sickness (CMS) who are venesected and studied for several weeks. In the final crossover period of the study, patients receive an iron infusion first followed by placebo (saline) infusion.
CMS - placebo firstNormal salinePatients with chronic mountain sickness (CMS) who are venesected and studied for several weeks. In the final crossover period of the study, patients receive a placebo (saline) infusion first followed by iron infusion.
CMS - ironNormal salinePatients with chronic mountain sickness (CMS) who are venesected and studied for several weeks. In the final crossover period of the study, patients receive an iron infusion first followed by placebo (saline) infusion.
CMS - ironIron sucrosePatients with chronic mountain sickness (CMS) who are venesected and studied for several weeks. In the final crossover period of the study, patients receive an iron infusion first followed by placebo (saline) infusion.
SLR - ironIron sucroseSea level residents (SLR) taken to high altitude for one week, and receiving iron infusion on Day 3 at high altitude.
Primary Outcome Measures
NameTimeMethod
Change in pulmonary artery systolic pressureOne week (SLR arm) and one month (CMS arm)
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Universidad Peruana Cayetano Heredia

🇵🇪

Lima, Peru

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