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Donor Lymphocyte Infusion After Stem Cell Transplant in Treating Patients With Haematological Cancers

Phase 2
Conditions
Lymphoma
Graft Versus Host Disease
Leukemia
Myeloma
Myelodysplastic Syndrome
Interventions
Other: No DLI
Other: CD4 DLI
Registration Number
NCT01240525
Lead Sponsor
University College, London
Brief Summary

RATIONALE: Giving low doses of chemotherapy, such as fludarabine and melphalan, before a donor stem cell transplant helps stop the growth of cancer cells. It also stops the patient's immune system from rejecting the donor's stem cells. The donated stem cells may replace the patient's immune cells and help destroy any remaining cancer cells (graft-versus-tumor effect). Giving an infusion of the donor's T cells (donor lymphocyte infusion) that have been treated in the laboratory after the transplant may help increase this effect. Sometimes the transplanted cells from a donor can also make an immune response against the body's normal cells. Giving alemtuzumab before transplant and cyclosporine after transplant, may stop this from happening.

PURPOSE: This randomized phase II trial is studying donor lymphocyte infusion after stem cell transplant in preventing cancer relapse or cancer progression in patients with follicular lymphoma, small lymphocytic non-Hodgkin lymphoma, or chronic lymphocytic leukemia.

Detailed Description

OBJECTIVES:

Primary

* To evaluate the effect of prophylactic transfer of donor CD4 cells after T-cell depleted reduced-intensity HLA-identical sibling transplantation upon the risk of relapse or progression in patients with haematological cancers (e.g. NHL, HL, CLL/PLL, PCM, AML, ALL, MDS or CMML depending on the disease status).

Secondary

* To evaluate the effect of prophylactic transfer of donor CD4 cells upon the risk of graft-versus-host disease (GvHD) in these patients.

* To evaluate the effect of prophylactic transfer of donor CD4 cells upon the rates of conversion to full donor chimerism in peripheral blood in these patients.

* To determine the effect of prophylactic transfer of donor CD4 cells upon immune reconstitution in these patients.

* To evaluate the impact of prophylactic transfer of donor CD4 cells upon non-relapse mortality and overall survival of these patients.

OUTLINE: This is a multicenter study.

Patients receive fludarabine IV, melphalan IV, and alemtuzumab IV as reduced intensity conditioning for T-cell depletion followed by a reduced-intensity HLA-identical sibling stem cell transplantation on day 0. Withdrawal of cyclosporine immunosuppression therapy commence at day 40 with tapering over a period of 3-4 weeks, according to the discretion of the PI. Patients are reassessed between day 70-90 post-transplantation. Patients with stable engraftment, no significant graft-versus-host disease, and no early relapse or progression are randomized to 1 of 2 treatment arms.

* Arm I: Patients receive an allogeneic CD4 donor lymphocyte infusion (DLI) at a dose of 1 x10\^6 CD4 cells/kg body weight without any other medication once between day 100-120.

* Arm II: Patients receive no further treatment.

Patients undergo blood sample collection for chimerism studies and translational research.

After completion of study treatment, patients are followed up periodically for 1 years and then annually.

Peer Reviewed and Funded or Endorsed by Bloodwise.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
114
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
No DLINo DLIPatients will receive no DLI post transplant as trial treatment.
CD4 DLICD4 DLIPatients will receive trial product manipulated CD4 DLI post transplant as trial treatment.
Primary Outcome Measures
NameTimeMethod
Progression-free survival at 1 year post-transplantduring the study and end of study
Secondary Outcome Measures
NameTimeMethod
Incidence, grade, or pattern of graft-versus-host diseaseduring the study and end of study
Rate of reconstitution of T-cell subsets and viral-specific immunityEnd of study
Cumulative incidence of non-relapse mortality at 1 yearEnd of study
Proportion of patients attaining multi-lineage full donor chimerism in peripheral bloodEnd of study
Incidence of infection requiring inpatient treatmentduring the study and end of study
Overall survival and non-relapse mortalityEnd of study

Trial Locations

Locations (11)

Birmingham Heartlands Hospital

🇬🇧

Birmingham, United Kingdom

Bristol Royal Hospital for Children

🇬🇧

Bristol, United Kingdom

Addenbrooke's Hospital

🇬🇧

Cambridge, United Kingdom

Beatson West of Scotland Cancer Centre

🇬🇧

Glasgow, United Kingdom

St James's University Hospital

🇬🇧

Leeds, United Kingdom

Leicester Royal Infirmary

🇬🇧

Leicester, United Kingdom

University College Hospital London (UCLH)

🇬🇧

London, United Kingdom

Royal Hallamshire Hospital

🇬🇧

Sheffield, United Kingdom

Nottingham City Hospital

🇬🇧

Nottingham, United Kingdom

University Hospitals Southampton

🇬🇧

Southampton, United Kingdom

Christie Hospital

🇬🇧

Manchester, United Kingdom

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