Variation of Genes Controlling Carbohydrate and Lipid Metabolism

Recruiting

• Conditions: Lipid Metabolism Disorders; Cardiovascular Diseases; Obesity; PreDiabetes; Diabetes Mellitus, Type 2; Insulin Resistance

• Registration Number: NCT03310502
• Lead Sponsor: Turku University Hospital

Brief Summary

Aim of the study is to investigate genes regulating glucose and lipid metabolism in subjects whose glucose metabolism, lipid metabolism, blood flow, or body fat distribution has been measured using positron emission tomography (PET), magnetic resonance imaging (MRI), magnetic resonance spectroscopy (MRS) or computed tomography (CT) as part of their previous participation in clinical trials conducted at Turku PET Centre.

By combining information from PET, MRI, CT, proteomics, metabolomics and genetics analyses we aim to find connection between genetic variation and metabolic and cardiovascular disease.

Detailed Description

The data is collected as a separate part of the clinical imaging study protocols using a separate inform consent approved by the local ethics committee. The participation for this study consists of drawing whole blood, serum and plasma samples for genetics, proteomics and metabolomics analyses. The results from these studies are combined with baseline data from the original clinical imaging studies to find connections between genetic variation, metabolism, blood flow and body fat distribution in a cross-sectional setting.

Study Timeline

• Study Start: 2002-04-17
• Study First Submitted: 2017-09-21
• Study First Submitted QC: 2017-10-10
• Study First Posted: 2017-10-16
• Status Verified: 2021-10
• Last Update Submitted: 2021-10-12
• Last Update Posted: 2021-10-13
• Primary Completion: 2022-12-31
• Study Completion: 2024-12-31

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 2050

Inclusion Criteria

• Previous participation of studies measuring glucose metabolism, lipid metabolism, bloow flow and adiposity at Turku PET Centre

Exclusion Criteria

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Skeletal muscle insulin sensitivity	Baseline	Skeletal muscle glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)

Secondary Outcome Measures

Name	Time	Method
Liver insulin sensitivity to glucose uptake	Baseline	Liver glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)
Abdominal subcutaneous adipose tissue mass	Baseline	Abdominal subcutaneous adipose tissue mass (kg)
Myocardial fatty acid uptake	Baseline	Myocardial fatty acid uptake at fasting state (µmol/kg tissue/min)
Liver fat content	Baseline	Liver fat percentage (%)
Brown adipose tissue glucose uptake during cold exposure	Baseline	Brown adipose tissue glucose uptake during cold exposure (µmol/kg tissue/min)
Abdominal subcutaneous adipose tissue insulin sensitivity to glucose uptake	Baseline	Abdominal subcutaneous adipose tissue glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)
Abdominal subcutaneous adipose tissue fatty acid uptake	Baseline	Abdominal subcutaneous adipose tissue fatty acid uptake at fasting state (µmol/kg tissue/min)
Brown adipose tissue fatty acid uptake at fasting state	Baseline	Brown adipose tissue fatty acid uptake at fasting state (µmol/kg tissue/min)
Insulin sensitivity of adipose tissue lipolysis	Baseline	Plasma free fatty acid concentration during euglycemic-hyperinsulinemic clamp (mmol/l)
Myocardial insulin sensitivity	Baseline	Myocardial glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)
Endogenous glucose production	Baseline	Endogenous glucose production during euglycemic-hyperinsulinemic clamp (µmol/kg body weight/min)
Visceral adipose tissue mass	Baseline	Visceral adipose tissue mass (kg)
Coronary flow reserve	Baseline	Ratio of myocardial blood flow maximum at stress (ml/g/min) vs. myocardial blood flow at rest (ml/g/min)
Whole body insulin sensitivity	Baseline	Whole body glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg body weight/min)
Visceral adipose tissue insulin sensitivity to glucose uptake	Baseline	Visceral adipose tissue glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)
Brown adipose tissue insulin sensitivity	Baseline	Brown adipose tissue glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)
Brain insulin sensitivity to glucose uptake	Baseline	Brain glucose uptake during euglycemic-hyperinsulinemic clamp (µmol/kg tissue/min)
Liver fatty acid uptake	Baseline	Liver fatty acid uptake at fasting state (µmol/kg tissue/min)
Visceral adipose tissue fatty acid uptake	Baseline	Visceral adipose tissue fatty acid uptake at fasting state (µmol/kg tissue/min)
Brown adipose tissue fatty acid uptake during cold exposure	Baseline	Brown adipose tissue fatty acid uptake at cold exposure (µmol/kg tissue/min)
Skeletal muscle fatty acid uptake	Baseline	Skeletal muscle fatty acid uptake at fasting state (µmol/kg tissue/min)

Trial Locations

Locations (1)

Turku PET Centre (Turku University Hospital); 🇫🇮 Turku, Finland