HO-1 and Muscle Mitochondrial Dysfunction in Sepsis

• Conditions: Sepsis; Atrophy
• Interventions: Procedure: therapy according to SSC guidelines and muscle biopsy; Procedure: standard surgery for femoral fracture and muscle biopsy

• Registration Number: NCT02359240
• Lead Sponsor: Changhai Hospital

Brief Summary

The current project was designed to examine dynamic changes in muscle wasting during sepsis. Researchers will focus the mitochondrial dysfunction of muscle cells and investigate the role of HO-1 in it. Researchers interested in identifying factors involved in the pathology of muscle wasting during sepsis.

Detailed Description

Rapid muscle atrophy or muscle wasting occured to septic patients.Sepsis induced muscle wasting has become a severe, frequent, and persistent complication among critically ill patients. Multiple factors including ubiquitin-proteasome system, proinflammatory cytokines and oxidative stress contribute muscle proteolysis.

It is known that heme oxygenase-1 (HO-1) possesses a variety of functions like anti-inflammatory, antioxidative and anti-apoptosis effects. In order to investigate the mechanisms under the condition, researchers are going to examine the role of HO-1 in muscle atrophy during sepsis.

Study Timeline

• Study Start: 2014-12
• Study First Submitted: 2015-01-28
• Status Verified: 2015-02
• Study First Submitted QC: 2015-02-04
• Last Update Submitted: 2015-02-04
• Study First Posted: 2015-02-09
• Last Update Posted: 2015-02-09
• Primary Completion: 2016-06
• Study Completion: 2016-08

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 50

Inclusion Criteria

• sepsis, severe sepsis, septic shock
• clinical reason for fixation surgery of femoral fractures

Exclusion Criteria

• no informed consent
• medical history of myopathy
• unintended weight loss before surgery or ICU admission
• chronic use of corticosteroids

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
sepsis	therapy according to SSC guidelines and muscle biopsy	Patients with a diagnosis of sepsis or severe sepsis,immobilization for 3 days at least. Therapy according to the SSC guidelines will be applied. therapy according to SSC guidelines and muscle biopsy
Patients with femoral fractures	standard surgery for femoral fracture and muscle biopsy	non septic patients with a femoral fracture,who need an fixation surgery.standard surgery for femoral fracture and muscle biopsy will be performed.

Primary Outcome Measures

Name	Time	Method
HO-1 mRNA and protein expression, mitochondria function of musculus vastus lateralis	3 days after diagnosis

Secondary Outcome Measures

Name	Time	Method
length of ICU stay	60 days
muscle myosin content	3 days after diagnosis
length of mechanical ventilation	60 days

Trial Locations

Locations (1)

Anesthesiology and Intensive Care, Changhai Hospital; 🇨🇳 Shanghai, Shanghai, China