Forearm Immobilization in T2D

Not Applicable

Recruiting

• Conditions: Healthy; Type 2 Diabetes

• Registration Number: NCT06750497
• Lead Sponsor: Wageningen University

Brief Summary

The aim of the present study is to assess the impact of short-term forearm immobilization on forearm muscle glucose uptake and amino acid net balance and kinetics in individuals with T2D compared with a control group with normoglycaemia.

Detailed Description

Participants with and without T2D will undergo 2 days of forearm immobilization as a model of local physical inactivity. Before, throughout, and after immobilization arteriovenous forearm balance measurements will be combined with a stable isotope tracer infusion to measure muscle glucose uptake and amino acid balance, uptake, and efflux.

Study Timeline

• Study Start: 2024-11-21
• Study First Submitted: 2024-11-22
• Study First Submitted QC: 2024-12-19
• Study First Posted: 2024-12-27
• Status Verified: 2025-07
• Last Update Submitted: 2025-07-14
• Last Update Posted: 2025-07-15
• Primary Completion: 2026-04-30
• Study Completion: 2026-04-30

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 26

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Change in forearm muscle glucose uptake	From 0 to 48 hours	The change in postprandial muscle insulin sensitivity, measured as forearm glucose uptake, from pre-immobilization to post-immobilization

Secondary Outcome Measures

Name	Time	Method
Temporal change in forearm glucose uptake	Prior to and for 3.5 hours following every meal consumption during the 48 hours forearm immobilization period	The change in postprandial muscle insulin sensitivity, measured as the temporal response of forearm glucose uptake (FGU), in response to repeated meals during 48 hours of forearm immobilization
Change in forearm muscle amino acid kinetics	Baseline and at regular intervals for 3.5 hours following the first and last meal consumption during the test period (0 to 48 hours)	Plasma phenylalanine kinetics assessed using a stable isotope tracer infusion. Measurements include the net balance (NB) of phenylalanine across the forearm.
Change in whole-body substrate oxidation	Measurements will be performed at baseline, and at postprandial intervals (at 1 hour, 2 hours, 3 hours) during a 0 to 48-hour test period.	Change in whole-body substrate oxidation measured using indirect calorimetry, measured before and in response to repeated mixed meals

Trial Locations

Locations (1)

Wageningen University and Research; 🇳🇱 Wageningen, Gelderland, Netherlands