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Safety, Tolerability and Clinical Activity of ASM-024 in Subjects With Mild Allergic Asthma

Phase 2
Completed
Conditions
Mild Allergic Asthma
Interventions
Drug: Placebo
Registration Number
NCT01092403
Lead Sponsor
Asmacure Ltée
Brief Summary

The study will assess the safety, tolerability and clinical activity of ASM-024 in subjects with mild allergic asthma.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
24
Inclusion Criteria
  • Able and willing to provide written informed consent;

  • Male or female subjects, ≥18 years and ≤ 50 years of age;

  • Female subjects of childbearing potential must have a negative pregnancy test (serum b-HCG) at Pre-Screening, and a negative urine pregnancy test immediately before the first administration of the study drug for each of the three Treatment Periods. Sexually active females must be willing to use adequate contraception.

  • Male subjects must be willing to use a condom with a spermicide for the duration of their participation in the study, plus an additional 30 days following study drug administration and ensure that their partner is using a highly effective method of birth control such as combined oral contraceptives, implants, injectables or a IUD. Male subjects must ensure that their female partner is willing to use adequate contraception;

  • Diagnosis of mild allergic asthma that meets the following criteria:

    • Stable on inhaled short-acting beta-2-agonists p.r.n. as the only medication for asthma.
    • Presence of both early asthmatic response (EAR) (at least 20 % fall in FEV1 within 3 hours after allergen inhalation) and late asthmatic response (LAR) (at least 15 % fall in FEV1).
    • Baseline methacholine (PC20) ≤ 16 mg/mL.
  • FEV1 of at least 70 % of the predicted value at Pre-Screening and Screening / Baseline;

  • BMI ≥ 19 and ≤ 35 kg/m²;

  • Body weight ≥ 40 kg;

  • Positive skin prick test to at least one common aeroallergen.

Exclusion Criteria
  • Any lung disease other than mild allergic asthma;

  • Pregnant or nursing women or women intending to conceive during the course of the study or have a positive serum pregnancy test at Pre-Screening or a positive urine pregnancy test during the study;

  • Women of childbearing potential (unless surgically sterilized by hysterectomy or bilateral tubal ligation, or post-menopausal for at least two years) not using a highly effective method of birth control. Highly effective methods of birth control are defined as those which result in a low failure rate (i.e., less than 1 % per year) when used consistently and correctly such as implants, injectables, combined oral contraceptives, intra-uterine devices (IUDs), sexual abstinence or a partner who has undergone a vasectomy;

  • Respiratory tract infections or worsening of asthma within 6 weeks before Screening/Baseline;

  • Baseline methacholine PC20 > 16 mg/mL at Screening / Baseline;

  • Current cigarette smokers or former smokers with a smoking history of greater than 10 pack years or who stopped smoking within the 12 months preceding enrolment in the study;

  • Use of any nicotine containing products within 6 months before Pre-Screening;

  • Any of the following concomitant medications:

    • Any medication that are known to prolong QT / QTc interval.
    • Oral or inhaled corticosteroids within 28 days preceding Pre-Screening or systemic corticosteroids within 90 days of Pre-Screening.
    • Long acting beta-2-agonists within one week preceding Baseline.
    • Use of inhaled short-acting β2- agonists or anticholinergics within 8 hours before all study visits to the clinic.
  • Known or suspected allergy or sensitivity to nicotine or cholinergic drugs or any drug with similar chemical structure;

  • Clinically significant ECG abnormalities at Pre-Screening including clinically significant or marked baseline prolongation of QT / QTc interval (e.g. repeated demonstration of a QTc interval of > 450 ms). Other non clinically significant findings such as sinus bradycardia, sinus arrhythmia, borderline first degree AV block (up to 205 ms), left ventricular hypertrophy (on voltage criteria for a subject less than 40 years old for instance) are permissible if judged to be acceptable by the Qualified investigator;

  • Family history of additional risk factors for TdP (e.g., family history of Long QT Syndrome.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboPlacebo once daily by inhalation
ASM-024ASM-024ASM-024 once daily by inhalation
Primary Outcome Measures
NameTimeMethod
Late asthmatic response (LAR)Day 8 of each treatment period

LAR as measured by the peak drop in FEV1 from 3 to 7 hours post-allergen challenge

Early asthmatic response (EAR)Day 8 of every treatment period

EAR as measured by the peak drop in FEV1 from 0 to 3 hours post-allergen challenge

Airway hyperresponsivenessDays -1, 7 and 9 of each treatment period

Difference between methacholine PC20 measured 24 hours following allergen challenge and methacholine PC20 measured 24 hours before allergen challenge

Safety and tolerabilityPhysical examination: Day 9, vital signs: Days -1, 1, 7, 8 and 9; twelve-lead ECG: Days 1, 7, 8 and 9 , AEs throughout the study, safety laboratory assessments Day 1 and 9 and Chest X-Ray: Day 9 of the final treatment period
Secondary Outcome Measures
NameTimeMethod
LAR's FEV1 AUCDay 8 of every treatment period

From 3 to 7 hours post-allergen challenge

FEV1Day 9

24 hours post-allergen challenge

EAR's FEV1 AUCDay 8

From 0 to 3 hours post-allergen challenge

Change in FEV1Days 1, 7, 8 and 9

Before inhalation of ASM-024 and as soon as possible following inhalation of ASM-024

Induced sputum eosinophil count and eosinophil and neutrophil percentagesDays -1, 7 and 9 of every Treatment Period
Blood eosinophil countDays -1 and 9 of every Treatment Period
Total and differential WBC countDays -1 and 9 of every Treatment Period

Trial Locations

Locations (3)

University of Saskatechewan

🇨🇦

Saskatoon, Saskatchewan, Canada

Mc Master University Health Sciences Center

🇨🇦

Hamilton, Quebec, Canada

Centre de Recherche - Institut universitaire de cardiologie et de pneumologie de Québec

🇨🇦

Québec, Quebec, Canada

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