A case-control study of oral and gut microbiota, malnutrition and appetite in community-dwelling older adults in the Netherlands

Completed

• Conditions: Malnutrition; 10003018

• Registration Number: NL-OMON47862
• Lead Sponsor: Vrije Universiteit Medisch Centrum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 28 February 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 400

Inclusion Criteria

Age 65 years and older;
Mentally competent to agree with and participate in study;
Living at home (non-institutionalized);
Participant of the LASA-cohort (Longitudinal Aging Study Amsterdam).

Exclusion Criteria

Body-Mass Index of 30 and above;
Active malignancy (with the exception of basal cell carcinoma);
History of inflammatory bowel disease;
Immune suppression (either medicinal or disease-related);
Use of antibiotics three months prior to faecal and salivary sampling;
Long-term admittance (more than 4 weeks) to hospital or long-term care facility in three months prior to faecal and salivary sampling;
Clinical symptoms of acute gastro-enteritis in the two weeks prior to faecal and salivary sampling (i.e. vomiting, acute diarrhoea).

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Main study parameter will be the intestinal microbial composition as determined<br /><br>by 16S RNA-sequencing of frozen faecal samples. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>We will investigate the oral microbiota as determined by 16S RNA-sequencing of<br /><br>frozen, tongue swabs and salivary samples. We will further determine metabolic,<br /><br>endocrine and inflammatory biomarkers from faecal, salivary and blood samples<br /><br>to investigate the different pathways through which the microbiota influences<br /><br>its host. We will also study the differences in oral microbiota and the<br /><br>relationship with subjects* sense of taste and smell as these might also<br /><br>influence loss of appetite. </p><br>