Muscle Memory After Treatment With Anabolic Substance Clenbuterol and Resistance Training in Humans: Myonuclear Addition, Hypertrophy and Myocellular Reprogramming
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Skeletal muscle proteome
研究概览
简要总结
The purpose of the project is investigate muscle memory and underlying mechanisms in humans following resistance training and use of the anabolic substance clenbuterol.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy men and women
- •BMI of <26 and normal ECG and blood pressure
排除标准
- •Chronic disease,
- •Use of prescription medication
- •Pain due to current or previous musculoskeletal injury
- •Resistance training more than once per week in the 12 months leading up to the intervention
- •Current or previous use of prohibited anabolic substances
研究组 & 干预措施
Clenbuterol
Participants are randomized to daily ingestion of clenbuterol for a period of 8 weeks, with or without supervised resistance training.
干预措施: Clenbuterol (Drug)
Placebo
Participants are randomized to daily ingestion of placebo for a period of 8 weeks, with or without supervised resistance training.
干预措施: Placebo (Drug)
结局指标
主要结局
Skeletal muscle proteome
时间窗: The change in the muscle proteome is assessed at baseline, 8, 24 and 32 weeks
Pathway enrichment analysis of the muscle proteome using mass-spectrometry based proteomics
Myonuclei
时间窗: The change in myonuclei is assessed at baseline, 8, 24 and 32 weeks
Immunohistochemistry to determine number of myonuclei
Fiber cross-sectional area
时间窗: The change in fiber cross-sectional is assessed at baseline, 8, 24 and 32 weeks
Immunohistochemistry to determine fiber cross-sectional area
次要结局
- Lean mass(The change in lean mass is assessed at baseline, 8, 24 and 32 weeks)
- Satellite cells(The change in satellite cells is assessed at baseline, 8, 24 and 32 weeks)
- Dynamic muscle strength(The change in 1-repetition maximum is assessed at baseline, 8, 24 and 32 weeks)
- Isometric muscle strength(The change in maximal voluntary isometric contraction is assessed at baseline, 8, 24 and 32 weeks)
- Sprint performance(The change in sprint performance is assessed at baseline, 8, 24 and 32 weeks)
研究者
Morten Hostrup, PhD
Associate Professor
University of Copenhagen