Antimicrobial Efficacy of Nano-Based Intracanal Medications on Enterococcus Faecalis
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Sponsor
- Tanta University
- Enrollment
- 30
- Locations
- 1
- Primary Endpoint
- the total numbers of Enterococcus Faecalis bacteria on bile esculin agar
Study Overview
Brief Summary
This in-vivo study aims to evaluate the antimicrobial effect of nano based intracanal medications (Triple antibiotic-loaded chitosan nanoparticles and chlorohexidine loaded by silver nanoparticles) on Enterococcus faecalis count reduction in secondary endodontic infection cases.
Detailed Description
Success of root canal treatment is largely dependent on the elimination of harmful microorganisms and primarily bacteria from the root canal system through chemomechanical preparation and using intracanal medication in order to cure or prevent periapical disease and allowing the maintenance of the dental element.
Failure of endodontically treated teeth is multifactorial; it is believed that the most important reason for endodontic treatment failure is the presence of microorganisms in the apical third of the root canal that survive after endodontic procedures.
Enterococcus faecalis (E. faecalis) is among the most commonly isolated bacteria from the failed endodontically treated root canals. Enterococcus is a facultative anaerobic gram-positive coccus that invades dentinal tubules, can survive in alkaline environment, endure prolonged periods of starvation, and have certain virulence factors and lytic enzymes. Its prevalence in primary endodontic infection ranges from 4% to 40 % and secondary endodontic infection ranges from 24% to 77%.
Moreover, anatomical complexity and diversity of root canals that hinders a complete disinfection of root canal space by chemo-mechanical instrumentation alone. So, the use of an intracanal medication remains an important adjunct to complete elimination of remaining bacteria by reaching areas that instruments and irrigation could not reach in addition to long-standing infections.
So, using of intracanal medication is mandatory in infected endodontic cases as more thorough debridement is achieved because of the longer overall time used for the treatment.It prevents regrowth of the remaining microbiota within the root canal system and also to increase the level of disinfection.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Treatment
- Masking
- Double (Participant, Care Provider)
Eligibility Criteria
- Ages
- 19 Years to 60 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Single rooted, single canal premolar teeth.
- •Teeth indicated for non-surgical root canal retreatment.
- •Teeth have signs and/or symptoms of post-treatment disease manifested by recurrent acute and/or chronic periapical abscess.
- •Teeth have clinical signs and symptoms of endodontic failure as sensitivity to percussion, pain, swelling or fistula.
- •Teeth with radiographic features of endodontic failure as persistent periapical lesion or widening of periodontal ligament.
- •Symptomatic or asymptomatic teeth with short root canal filling indicated for prosthetic restoration
Exclusion Criteria
- •Patients with any systemic diseases.
- •Pregnant or lactating patients.
- •Immunocompromised patients.
- •Non-restorable tooth.
- •Calcified root canal.
- •Teeth with root fracture.
- •Teeth with periodontal pocket deeper than 4mm.
- •Teeth have procedural error like ledge, broken instrument, or perforation
Outcomes
Primary Outcomes
the total numbers of Enterococcus Faecalis bacteria on bile esculin agar
Time Frame: one weak after application of intracanal medication
colony forming units (CFU/ml).
Secondary Outcomes
- Post operative pain(one weak after application of intracanal medication)
Investigators
Ibrahim Ali Eissa
doctor
Tanta University
