The effects of acute exercise on insulin sensitivity, bone metabolism and cardiovascular risk factors in healthy young men treated with a single dose of prednisolone

Not Applicable

Completed

• Conditions: Insulin sensitivity; Markers of bone remodelling; Markers of inflammation; Mitochondria function; Musculoskeletal - Other muscular and skeletal disorders; Metabolic and Endocrine - Metabolic disorders

• Registration Number: ACTRN12615000755538
• Lead Sponsor: Itamar Levinger

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2015-07-21
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Completed
• Sex: Male
• Target Recruitment: 12

Inclusion Criteria

Healthy men aged 18-40 years. BMI 19-27 kg/m2. Fasting blood glucose equal or less than 5.6 mmol/L

Exclusion Criteria

- Men with bone disease (such as osteoporosis)
- Men who are taking any prescribed medication known to affect bone metabolism, insulin secretion or insulin sensitivity.
- Musculoskeletal or orthopaedic condition (such as severe osteoarthritis) that prevents normal daily function (such as walking).
- Men with metabolic or cardiovascular disease
- Volunteers on warfarin therapy or vitamin K supplementation or restriction will be excluded

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Changes in bone remodelling markers including: total osteocalcin, uncarboxylated osteocalcin, procollagen type 1 N-terminal propeptide and beta-isomerized C-terminal telopeptides.<br><br>Blood samples will be collected from the antecubital vein using a venepuncture technique.[Baseline, post exercise, 30 min post exercise and 1, 2 and 3 hours post exercise and every 30 minutes during the 2 hour insulin clamp.]

Secondary Outcome Measures

Name	Time	Method
Changes in insulin sensitivity[Post insulin clamp. Will be assessed using the Glucose Infusion Rate (GIR) in the last 30 min of the clamp.];Changes in skeletal muscle metabolism. Will be assessed using a muscle biopsy technique, with suction. Insulin signalling proteins and mitochondria function will be examined. [Baseline, 3 hours post exercise and post 2 hour of insulin clamp.];Changes in markers of cytokines in blood and skeletal muscle. Including IL6, TNF-alpha, MCP1, IL1-beta, atrogin, STAT3, NF-k beta, FoxO1, MuRF1.<br>Blood samples will be collected from the antecubital vein using a venepuncture technique<br>Muscle sample: will be taken using muscle biopsy technique, with suction.[Baseline, 3 hours post exercise and post 2 hour of insulin clamp.]