Effect of Spirulina on the Levels of Zinc, Vitamin E and Linoleic Acid in the Palm Skin Extracts of People With Prolonged Exposure to Arsenic
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Change in vitamin E levels in skin palm extract following supplementation with spirulina
研究概览
简要总结
Patients of arsenical keratosis may be treated with spirulina. Is this improvement related to the levels of zinc, vitamin E and linoleic acid at the site of the keratosis (palm)? To understand this, patients of palmer arsenical keratosis (n=10), arsenic exposed controls (n=10) and healthy volunteers (n=10) will be treated with spirulina powder 10 g/day orally for 12 weeks. Skin extracts will be collected both before and after supplementation from the palm and dorsum for estimation of zinc, vitamin E and linoleic acid levels.
详细描述
Patients of arsenical keratosis remain untreated due to unavailability of effective drug. To find out an effective drug, the pathogenesis of keratosis should be understood. Spirulina is one of the dietary supplements, that improves the symptoms of keratosis. Is this improvement related to the levels of zinc, vitamin E and linoleic acid at the site of the keratosis (palm)? Ten patients of moderate palmer arsenical keratosis will be treated with spirulina powder 10 g/day orally for 12 weeks. Skin extracts will be collected both before and after supplementation from the palm and dorsum using a mixture of chloroform and ethanol. Zinc, vitamin E and linoleic acid levels will be estimated from the extracts. Similar protocol of spirulina administration and the collection of skin extracts will be done in arsenic exposed controls (n=10) and healthy volunteers (n=10).
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •(Patients):
- •moderate palmer keratosis
- •drink arsenic contaminated water (>50 µg/L) for at least 6 months
- •voluntarily agree to participate
- •Inclusion Criteria (Arsenic exposed controls):
- •no symptoms of keratosis
- •family member of the patient
- •drink arsenic contaminated water (>50 µg/L) for at least 6 months
- •voluntarily agree to participate
- •Inclusion Criteria (Healthy volunteers):
- •no symptoms of keratosis
- •drink arsenic safe water (<50 µg/L)
- •voluntarily agree to participate
排除标准
- •pregnancy
- •lactation
- •chronic diseases: diabetes mellitus, rheumatoid arthritis, tuberculosis
- •arsenicosis patients under treatment in the previous 3 months
结局指标
主要结局
Change in vitamin E levels in skin palm extract following supplementation with spirulina
时间窗: 0 week (baseline), 12 weeks (end)
次要结局
- Change in zinc levels in skin palm extract following supplementation with spirulina(0 week (baseline), 12 weeks (end))
- Change in linoleic acid levels in skin palm extract following supplementation with spirulina(0 week (baseline), 12 weeks (end))
研究者
Prof. Mir Misbahuddin
Prof. and Chairman
Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh