Assessment of the Relationship Between Pain, Central Sensitization and Psychoemotional State in Patients With Chronic Masticatory Muscle Pain
Overview
- Phase
- Not Applicable
- Intervention
- Diagnostic Criteria for Temporomandibular Disorders Clinical Examination
- Conditions
- Not specified
- Sponsor
- Wroclaw Medical University
- Enrollment
- 102
- Locations
- 1
- Primary Endpoint
- Relationship between masticatory muscle pain and polimorphism of serotonin receptor gene HTR2A - rs4941573 and dopamine receptor gene DRD3 - rs6280
- Status
- Enrolling By Invitation
- Last Updated
- 2 months ago
Overview
Brief Summary
The aim of this study is to investigate the relationship of psychological aspects, selected blood parameters, pain, central sensitization, and muscle electromyography activity in patients with chronic masticatory muscle pain.
Detailed Description
In this prospective cohort study, the study group will consist of 51 people with masticatory muscle pain lasting longer than 3 months, while the control group will involve 51 people without such disorder. Muscle type of temporomandibular disorders will be diagnosed on the basis of validated and standardized DC/TMD protocol. Additionally potential headaches will be assessed according to the ICHD-3, since coexistence of chronic masticatory muscle pain and headache is often observed. Psychological aspects will be analyzed using validated questionnaires regarding depression (PHQ-9), anxiety (GAD-7), perceived stress (PSS-10), satisfaction with life (SWLS), type D personality (DS-14) and coping with stress (Mini-COPE). Central Sensitization will be assessed using the Central Sensitization Inventory. Following blood substances will be assessed: serotonin, dopamine, Gamma-aminobutyric acid (GABA), Tumor Necrosis Factor α (TNF-α), CGRP, SERT, interleukin 1B, interleukin 6, interleukin 8, interleukin 10 and interleukin 17A. Additionally two polymorphisms of genes encoding proteins for serotonin receptors HTR2A - rs4941573 in intron 3 and dopamine DRD3 - rs6280 (Ser9Gly) will be analyzed. Pain assessment will be done using a validated and standardized questionnaire regarding chronic pain (GCPS) and an algometer and will include the measurement of pressure pain threshold in masseter and temporal muscles. In addition, potential autonomic disorders will be assessed using Autonomic Symptoms Questionnaire proposed by Low. Electromyographic muscle activity will be assessed by tetany test. The results of the study will allow for deeper understanding of the complex nature of orofacial pain of muscle origin and its connection with the mechanism of central sensitization, headache and muscle electromyographic activity. Measurement of blood parameters will provide more accurate information on the pathophysiology of these disorders and the use of validated questionnaires will provide valuable information about the mental state of patients.
Investigators
Joanna Smardz
Investigator
Wroclaw Medical University
Eligibility Criteria
Inclusion Criteria
- •for study group:
- •Masticatory muscle pain for at least 3 months, based on DC/TMD, at least 18 y.o
Exclusion Criteria
- •severe neurological or psychiatric diseases, use of drugs that may affect the neuromuscular system, alcohol or drug addiction, cancer or pregnancy, previous head surgery
- •Inclusion criteria for control group:
- •no pain in masticatory muscles, age of at least 18 y.o Exclusion criteria the same as for study group
Arms & Interventions
Study group
Participants with masticatory muscle pain lasting for over 3 months.
Intervention: Diagnostic Criteria for Temporomandibular Disorders Clinical Examination
Study group
Participants with masticatory muscle pain lasting for over 3 months.
Intervention: Blood test
Control group
Participants without masticatory muscle pain in last 6 months.
Intervention: Diagnostic Criteria for Temporomandibular Disorders Clinical Examination
Control group
Participants without masticatory muscle pain in last 6 months.
Intervention: Blood test
Outcomes
Primary Outcomes
Relationship between masticatory muscle pain and polimorphism of serotonin receptor gene HTR2A - rs4941573 and dopamine receptor gene DRD3 - rs6280
Time Frame: January 3, 2023 - January 3, 2024
Each participant will undergo genetical blood test.
Relationship between masticatory muscle pain and serotonin and dopamine levels.
Time Frame: January 3, 2023 - January 3, 2024
Each participant will undergo blood test.
Relationship between masticatory muscle pain and Gamma-aminobutyric acid (GABA), Tumor Necrosis Factor α (TNF-α), CGRP, SERT, interleukin 1B, interleukin 6, interleukin 8, interleukin 10 and interleukin 17A.
Time Frame: January 3, 2023 - January 3, 2024
Each participant will undergo blood test.
Secondary Outcomes
- Relationship between masticatory muscle pain and PPTs.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and anxiety assessed by Generalized Anxiety Disorder -7.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and perceived stress assessed by Perceived Stress Scale.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and depression assessed by Patient Health Questionnaire.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and satisfaction with life assessed by Satisfaction With Life Scale.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and Type D personality assessed by DS-14 quesstionaire.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and coping with stress assessed by mini COPE.(January 3, 2023 - January 3, 2024)
- Relationship between masticatory muscle pain and central sensitization assessed by Central Sensitization Inventory.(January 3, 2023 - January 3, 2024)