Relationship Between Oxidative Stress and Aortic Stiffness During Acute Exhaustive Training in Adolescent Football Players
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 43
- 试验地点
- 4
- 主要终点
- aortic strain (%)
研究概览
简要总结
The goal of this clinical trial is to search about if there is any relationship between the aortic stiffness and oxidative stress response in adolescent football players. The main questions are;
- Is there any difference between the adolescent football players and healthy sedentary control group in terms of oxidative stress and aortic stiffness parameters?
- Is there any relationship between the aortic stiffness and oxidative response to acute exercise training in adolescent male football players? Participants will perform acute exhaustive exercise training. Venous blood will be drained before and after exercise training. Oxidative parameters will be measured.
详细描述
Arterial stiffness is defined as stiffening of the arterial wall and refers to a decrease in the capacity of an artery to expand and contract in response to pressure changes in the vessel. The parameters indicating arterial stiffness are compliance and distensibility of the vessel.
Imbalance in the formation of vasodilator and vasoconstrictor signaling molecules acting on the vessel is an important determining factor in the development of endothelial dysfunction, which is often exacerbated by oxidative stress. Disruption of the balance between oxidants and antioxidants in favor of oxidants leads to disruption of the elastin-collagen ratio in the vessel wall in favor of collagen and ultimately to the development of arterial stiffness. Regular exercise is beneficial both to prevent the development of arterial stiffness and to reduce arterial stiffness. Although many mechanisms have been mentioned for exercise to improve endothelial function, there is no clear consensus on the mechanisms by which exercise increases arterial compliance, which is one of the indicators of atherosclerosis. While the beneficial impacts of physical activity on arterial stiffness are well-documented, this varies considerably among sports disciplines. For instance, there are studies showing that endurance training decreases arterial stiffness in young adults and elderly individuals, whereas strength training increases arterial stiffness. Arterial stiffness is lower in endurance athletes with a longer sports history and higher in those with a strength training history. The weekly training time of competitive young athletes is well beyond the amount of exercise required for healthy living. It has also been suggested that this excessive amount of time may trigger some unfavorable adaptations in the cardiovascular system. In addition, the number of studies examining the effects of regular exercise training at these levels on the vascular system is limited. Studies indicate that changes in arterial stiffness start at early ages and this is also true for young athletes who perform regular training in the infrastructures of various sports branches. Therefore, our aim is investigating the relationship between the oxidative stress parameters and aortic stiffness in adolescent male football players and comparing with the healthy control group. Secondly, the investigators will evaluate if there is any difference between the aortic stiffness and oxidative response to acute exercise training in adolescent male football players?
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Screening
- 盲法
- Double (Investigator, Outcomes Assessor)
盲法说明
Echocardiographer and biochemistry expert will be blind to participants type (sedentary or football player)
入排标准
- 年龄范围
- 14 Years 至 18 Years（Child, Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •age between 14-18
- •male gender
- •football player
- •attending regular football training for at least 1 season
排除标准
- •female gender
- •attending any other sports training except from football
- •having any chronic disease
- •having any musculoskeletal disease which limits to join exhaustive exercise training
结局指标
主要结局
aortic strain (%)
时间窗: will be measured twice before and after acute exhaustive exercise, through study completion, up to 8 weeks
=\[(aortic systolic diameter - diastolic diameter)× 100\] / aortic diastolic diameter
aortic stiffness index
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
= ln (systolic pressure / diastolic pressure) / aortic strain
aortic distensibility (cm2.dyn-1.10-6)
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
= (2 × aortic strain) / (systolic pressure-diastolic pressure)
serum lipid hydroperoxide assay
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
lipid hydroperoxide (LHP) level of serum (nmol/mL). Lipid hydroperoxide (LOOH) is the first, comparatively stable, product of the lipid peroxidation reaction.
serum malondialdehide assay
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
serum malondialdehide (MDA) level of serum (nmol/mL).MDA is the end product of the oxidation of polyunsaturated fatty acids.
serum advanced oxidation protein products assay
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
Advanced oxidation protein products (AOPP) level of serum (mikromol/L ). AOPP are the dityrosine-containing and crosslinking protein products formed during oxidative stress by reaction of plasma protein with chlorinated oxidants, and often carried by albumin in vivo
serum advanced glycation end products assay
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
serum advanced glycation end products (AGE) level of serum(FU/mg). AGEs are are a heterogeneous group of proteins and lipids, formed by nonenzymatic glycation after persistent contact with aldose sugars
serum ferric reducing antioxidant power assay
时间窗: will be measured twice before and after acute exhaustive exercise, through study completion, up to 8 weeks
ferric reducing antioxidant power (FRAP) level of serum (mikromol/mL ). FRAP is an antioxidant capacity assay that uses Trolox as a standard
serum superoxide dismutase activity assay
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
copper-zinc superoxide dismutase activity (SOD) level of serum (U/mL). SODs are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide
serum total antioxidant status and total oxidant status assay
时间窗: will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks
total antioxidant status (TAS) and total oxidant status (TOS) levels of serum (trolox equivalent/L) are two indicators to determine all antioxidants or oxidants in samples, which can more comprehensively reflect the changes of oxidant and antioxidant capacity of samples
次要结局
未报告次要终点
研究者
Şensu Dinçer
medical doctor
Istanbul University