Study on IgE Memory B cells in Allergic disease
Recruiting
- Conditions
- allergytype I hypersensitivity responses10001708
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 180
Inclusion Criteria
* children from 6-18 yrs old with asthma/rhinitis, atopic dermatitis or food allergy
* Atopy with proven allergen (RAST class >1.4 or specific IgE serum levels > 0.7 IU/L or positive skin prick test with HEP index > 0.21)
* Positive doubleblind food provocation test or convincing history of recent adverse response to food intake
* Informed written consent
Exclusion Criteria
- Patients < 6 yrs old
- Children who are receiving immunosuppressive therapy
- Children with substantial co-morbidity of immune-related diseases, especially immunodeficiencies or autoimmune disease (e.g. diabetes)
- Children receiving anti-IgE antibody treatment (e.g. with Xolair)
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>From each patient, the absolute B- and T-cell numbers in blood (cells per<br /><br>milliliter of blood) will be determined, as well as the relative frequencies of<br /><br>naïve and IgM+, IgG+, IgA+ and IgE+ memory B-cells. Total and allergen-specific<br /><br>serum IgE levels will be determined. These parameters will be compared between<br /><br>patients with food allergy, patients with asthma, patients with hay fever and<br /><br>healthy controls. Finally, we will study the correlation of IgE+ memory-B-cell<br /><br>numbers with serum IgE levels and the IgE allergen-specificity.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Relate the changes in the B-cell compartment and serum IgE levels to changes in<br /><br>T-cell subsets, eosinophils, basophils and mast cells in blood. Since the<br /><br>anatomical location differs between asthma/hay fever, atopic dermatitis and<br /><br>food allergy, we hypothesize that distinct B cells are involved.</p><br>