Biofilm Formation and Antibiotic Sensitivity Patterns

• Conditions: Orthopedic Surgery

• Registration Number: NCT05181163
• Lead Sponsor: Sohag University

Brief Summary

Detection of bacteria that form biofilm and its sensitivity to antibiotics in patients with surgical site infection after orthopedic surgery.

Detailed Description

The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to the following steps to identify the causative bacteria:

* Culturing on blood agar, chocolate agar, MacConkey agar and Mannitol salt agar , gram staining and biochemical tests.

* Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.

* Antimicrobial Susceptibility Testing using the Kirby-Bauer disk diffusion method.

Study Timeline

• Status Verified: 2021-12
• Study First Submitted: 2021-12-08
• Study First Submitted QC: 2021-12-19
• Last Update Submitted: 2021-12-19
• Study Start: 2022-01
• Study First Posted: 2022-01-06
• Last Update Posted: 2022-01-06
• Primary Completion: 2022-07
• Study Completion: 2023-01

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• patients with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...)

Exclusion Criteria

• if patient has no orthopedic surgery

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
detect biofilm formation	one year	The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.