In Vitro Biofilm Formation and Antibiotic Susceptibility Patterns of Bacteria From Surgical Site Infection After Orthopedic Surgery
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Orthopedic Surgery
- Sponsor
- Sohag University
- Enrollment
- 100
- Primary Endpoint
- detect biofilm formation
- Last Updated
- 4 years ago
Overview
Brief Summary
Detection of bacteria that form biofilm and its sensitivity to antibiotics in patients with surgical site infection after orthopedic surgery.
Detailed Description
The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to the following steps to identify the causative bacteria: * Culturing on blood agar, chocolate agar, MacConkey agar and Mannitol salt agar , gram staining and biochemical tests. * Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation. * Antimicrobial Susceptibility Testing using the Kirby-Bauer disk diffusion method.
Investigators
Amira Ahmed Talaat
Principal investigator
Sohag University
Eligibility Criteria
Inclusion Criteria
- •patients with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...)
Exclusion Criteria
- •if patient has no orthopedic surgery
Outcomes
Primary Outcomes
detect biofilm formation
Time Frame: one year
The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.