The Influence of Sperm Morphology and Functional Indices on Blastocyst Development in Assisted Reproduction
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 120
- 试验地点
- 1
- 主要终点
- Evaluation of sperm morphology indices on fertilization and blastulation rate
研究概览
简要总结
Samples from male partners of 120 infertile couples undergoing IVF were analyzed. Sperm morphology was assessed using the SpermBlue staining method, while TZI, MAI, and SDI were calculated. Embryo development was monitored until day 5 or 6. Statistical analyses included regression models and correlation coefficients to evaluate the predictive value of sperm indices on blastocyst development.
详细描述
For spermatozoa selection we used ZyMot multi in all patients undergoing IVF. After intracytoplasmatic injection of spermatozoa we asses the blastocyst grading in day 5. NEQSI score meaning numerical embryonic quality score index was used for statistical purpose. The spermatozoa morphology indexes were: TZI (teratozoospermia index), MAI (multiple anomalies index), SDI (sperm deformity index) and analyzed with CASA analyzer. All these indexes were assessed in patients and correlated in all groups: patients that had a positive pregnancy test, patients that had a negative pregnancy rate, patients that had no blastocyst in day 5 and patients that had all aneuploid embyos.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 21 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •couples with an infertility diagnosis that underwent an IVF procedure and had obtained at least one blastocyst
排除标准
- •uterine abnormalities
结局指标
主要结局
Evaluation of sperm morphology indices on fertilization and blastulation rate
时间窗: 18 months
Functional sperm indices and embryo outcomes were evaluated to determine their relationship.
次要结局
- Evaluation of sperm morphology indices on IVF outcomes(16 months)