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Acute Effects of Chemotherapy Administration on Skeletal Muscle of Breast Cancer Patients: the PROTECT-06 Study

Completed
Conditions
Breast Cancer
Interventions
Drug: Epirubicin-cyclophosphamide
Registration Number
NCT05128617
Lead Sponsor
Institut de cancérologie Strasbourg Europe
Brief Summary

Chemotherapy treatments such as epirubicin-cyclophosphamid or paclitaxel lead to severe off-target side effects such as skeletal muscle deconditioning. To date, three different studies investigated skeletal muscle decontioning in breast cancer patients, through long term protocols including all chemotherapy cycle treatment, and highlighted both structural alterations and impaired cellular processes. However, no study is currently availbale on the acute effect of one single chemotherapy administration in breast cancer patients skeletal muscle tissue. Our study is therefore dedicated to the investigation of the acute effect of the first dose administration of both Epuribicin/cyclophosphamide and Paclitaxel chemotherapies on skeletal muscle of breast cancer patients.

Detailed Description

Chemotherapy treatments such as epirubicin-cyclophosphamid or paclitaxel lead to severe off-target side effects such as skeletal muscle deconditioning. Resulting from a global perturbation of the muscle homeostasis, skeletal muscle is characterized by both structural and functional alterations that will translate into a decrease in muscle mass and/or force as well as an increase in muscle fatigability. These maladaptations result in a reduced quality of life and an increased treatment-related toxicity, ultimately leading to an increased mortality risk.To date, three different studies investigated skeletal muscle decontioning in breast cancer patients, through long term protocols including all chemotherapy cycle treatment, and highlighted both structural alterations and impaired cellular processes. However, no study is currently availbale on the acute effect of one single chemotherapy administration in breast cancer patients skeletal muscle tissue.Our study is therefore dedicated to the investigation of the acute effect of the first dose administration of both Epuribicin/cyclophosphamide and Paclitaxel chemotherapies on skeletal muscle of breast cancer patients. Our study will particularly explore cellular mechanisms of muscle decontioning, such as protein turnover, mitochondrial homeostasis and fatty infiltrations.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
20
Inclusion Criteria
  • Breast cancer (stade I to III)
  • Patient recieving the first administration of epirubicin-cyclophosphamide (group 1) or paclitaxel (group 2) for early breast cancer treatment
Exclusion Criteria
  • History of cancer
  • Known chronic pathology
  • Pacemaker
  • Contraindication to the evaluation of the physical condition
  • Contraindication to the local anesthesia for the muscle micro-biopsy
  • Breastfeeding or pregnant woman

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Epirubicin-cyclophosphamideEpirubicin-cyclophosphamideWoman with early breast cancer receiving a first cycle of epirubicin-cyclphosphamide
PaclitaxelPaclitaxelWoman with early breast cancer receiving a first cycle of paclitaxel
Primary Outcome Measures
NameTimeMethod
Investigate the acute effect of chemotherapy administration on protein turnover cellular processes through vastus lateralis biopsies of breast cancer patientsBefore and 4 days after the chemotherapy

Change From Baseline in Western Blots measurements of protein expression levels

Secondary Outcome Measures
NameTimeMethod
Investigate the acute effect of chemotherapy administration on structural alterationsBefore and 4 days after the chemotherapy

Change From Baseline in Cross-Sectional Area (CSA) measurements

Investigate the acute effect of chemotherapy administration on satellite cellsBefore and 4 days after the chemotherapy

Change From Baseline in Western Blot measurement of protein expression levels

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the muscle thicknessBefore and 4 days after the chemotherapy

Change From Baseline in ultrasonography measurement

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the appetite lossBefore and 4 days after the chemotherapy

Change From Baseline in FAACT auto-questionnaire measurement

Investigate the acute effect of chemotherapy administration on mitochondrial homeostasisBefore and 4 days after the chemotherapy

Change From Baseline in Western Blot measurement of protein expression levels

Investigate the acute effect of chemotherapy administration on fatty infiltrationsBefore and 4 days after the chemotherapy

Change From Baseline in Western Blot measurement of protein expression levels

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the muscle fibers fascicle lengthBefore and 4 days after the chemotherapy

Change From Baseline in ultrasonography measurement

Investigate the acute effect of chemotherapy administration on inflammationBefore and 4 days after the chemotherapy

Change From Baseline in Western Blots measurments of protein expression levels

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the physical activity levelBefore and 4 days after the chemotherapy

Change From Baseline in GPAQ auto-questionnaire measurement

.Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the skeletal muscle massBefore and 4 days after the chemotherapy

Change From Baseline in quantity of muscle mass measured by bioelectrical impedance analysis

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the muscle forceBefore and 4 days after the chemotherapy

Change From Baseline in the maximal isometric strength of the knee with force transducer

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the muscle fibers angle of pennationBefore and 4 days after the chemotherapy

Change From Baseline in ultrasonography measurement

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the echogenecityBefore and 4 days after the chemotherapy

Change From Baseline in ultrasonography measurement

Comparison of the acute effect of Epirubicin-Cyclophosphamide versus Paclitaxel on the quality of lifeBefore and 4 days after the chemotherapy

Change From Baseline in FACT-G auto-questionnaire measurement

Trial Locations

Locations (1)

Institut de Cancerologie Strasbourg Europe

🇫🇷

Strasbourg, France

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