Comparison of the in Vitro Development of Human Embryos in Static and and Microfluidics Systems of Culture
试验速览
- 阶段
- 2 期
- 发起方
- 入组人数
- 400
- 试验地点
- 2
- 主要终点
- Embryo morphology on days 2 and 3 of embryo culture according to the criteria described by Lucinda Veeck
研究概览
简要总结
Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.
详细描述
Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 盲法
- None
入排标准
- 年龄范围
- 21 Years 至 35 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Age between 21 and 35 years old
- •No more than 1 previous IVF failure
- •Absence of physical conditions that can interfere in oocyte quality
- •At least 8 zigotes 24 hours after fertilization
排除标准
- •Use of epididimary or testicular sperm
- •Sperm or egg donation
- •Untreated systemic diseases
结局指标
主要结局
Embryo morphology on days 2 and 3 of embryo culture according to the criteria described by Lucinda Veeck
时间窗: Embryo culture for 3 days before transference to the uterus
次要结局
- Implantation rate and pregnancy rate(24 month)