The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Delayed onset muscle soreness, DOMS (scale 1-10)
研究概览
简要总结
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
详细描述
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Supportive Care
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •subject provides written informed consent
- •physiological health profile
- •stable at their anthropometric characteristics for at least the last year
排除标准
- •muscle disease
- •prior history of musculoskeletal injury to the lower limbs
- •consumed any nutritional supplement the last 3 months
- •performed intense eccentric exercise the last 6 months
- •non Caucasian
研究组 & 干预措施
Young group
Participants will underwent two isokinetic eccentric exercise sessions
干预措施: Exercise (Other)
Elderly group
Participants will underwent two isokinetic eccentric exercise sessions
干预措施: Exercise (Other)
结局指标
主要结局
Delayed onset muscle soreness, DOMS (scale 1-10)
时间窗: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Muscle torque (torque)
时间窗: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Range of motion, ROM (degrees)
时间窗: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Creatine kinase, CK (activity IU)
时间窗: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
次要结局
- F2-isoprostanes (pg/mg creatinine)(Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Protein carbonyls (nmol/mg protein)(Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Glutathione, GSH (μmol/g Hb)(Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Catalase (U/g Hb)(Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Superoxide dismutase, SOD (U/g Hb)(Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Glutathione peroxidase, GPx (U/g Hb)(Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)(Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Uric acid (mM)(Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Albumin (μM)(Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Bilirubin (μM)(Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
研究者
Dr. Anastasios Theodorou
Researcher
European University Cyprus