Acute Effects of Pea Proteins With Different Degrees of Processing on Postprandial Metabolism in Healthy Women and Men
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Amino acid profile in blood
研究概览
简要总结
The aim of this study is to investigate the postprandial metabolic responses to plant-based protein ingredients of different degrees of processing (pea protein concentrate, isolate, and extrudate) and in comparison to whey protein in healthy adults. Therefore, young healthy subjects consume 4 test meals with 25 g of protein from pea protein concentrate, pea protein isolate, pea protein extrudate or whey protein in a randomized order. In a postprandial period of 6 hours, parameters of protein and glucose metabolism (i.a. plasma amino acids), gastric emptying and hunger/satiety are analysed. It is assumed that the plasma amino acid profile after plant protein ingestion differs depending on processing level of the protein ingredients and in comparison to whey protein.
详细描述
In a randomized crossover-design, 20 healthy women and men consume 4 test meals containing either 25 g of protein from plant protein ingredients (pea protein concentrate, isolate, or extrudate) or whey protein together with 1 g paracetamol, which is used as a marker for gastric emptying.
During a postprandial period of 6 hours parameters of protein metabolism (e.g. amino acids in plasma and urine), glucose metabolism (plasma glucose, serum insulin) and gastric emptying rate (based on plasma paracetamol) are analysed. Furthermore, subjective hunger/satiety and acceptance of the meals are assed using visual analogue scales. Each intervention arm will be separated by a washout period of at least 7 days.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •non-smoking
- •metabolically healthy
- •normal weight (BMI: 18,5 - 24,9 kg/m2)
- •written informed consent
排除标准
- •pregnancy, lactation
- •hypo- or hypertension
- •underweight or overweight/obesity
- •food intolerances and allergies (especially milk protein, pea, celery)
- •malabsorption syndromes
- •gastrointestinal diseases
- •thyroid diseases
- •diabetes mellitus type 1 and type 2
- •impaired kidney or liver function
- •blood coagulation disorders
- •irregular menstrual cycle
- •endometriosis, severe menstrual problems
- •hormonal contraception
- •regular use of medications (especially habitual use of paracetamol or medications that may interact with paracetamol)
- •hypersensitivity to paracetamol
- •body weight below 50 kg
- •alcohol abuse
- •glucose-6-phosphate dehydrogenase deficiency
- •Gilbert's syndrome
- •vegan diet
- •eating disorders (especially anorexia nervosa, bulimia nervosa)
- •participation in another study
结局指标
主要结局
Amino acid profile in blood
时间窗: Postprandial period of 5 hours (Time points: fasting (0) and 15, 30, 45, 60, 90, 120, 180, 240 and 300 minutes)
Analysis of amino acid profile in plasma (nmol/ml)
次要结局
- Parameters of protein metabolism in blood I(Postprandial period of 5 hours (Time points: fasting (0) and 30, 60, 90, 120, 180, 240 and 300 minutes))
- Parameters of protein metabolism in blood II(Postprandial period of 5 hours (Time points: fasting (0) and 60, 120, 180, 240 and 300 minutes))
- Parameters of glucose metabolism in blood I(Postprandial period of 5 hours (Time points: fasting (0) and 30, 60, 90, 120, 180, 240 and 300 minutes))
- Parameters of glucose metabolism in blood II(Postprandial period of 5 hours (Time points: fasting (0) and 30, 60, 90, 120, 180, 240 and 300 minutes))
- Gastric emptying rate(Postprandial period of 5 hours (Time points: fasting (0) and 30, 60, 90, 120, 180, 240 and 300 minutes))
- Zinc in blood(Postprandial period of 5 hours fasting (0) and 60, 120, 180, 240 and 300 minutes))
- Uric acid in blood(Postprandial period of 5 hours (Time points: fasting (0) and 30, 60, 90, 120, 180, 240 and 300 minutes))
- Copper in blood(Postprandial period of 5 hours fasting (0) and 60, 120, 180, 240 and 300 minutes))
- Iron in blood(Postprandial period of 5 hours fasting (0) and 60, 120, 180, 240 and 300 minutes))
- Selenium in blood(Postprandial period of 5 hours fasting (0) and 60, 120, 180, 240 and 300 minutes))
- Amino acid profile in urine(Postprandial period of 5 hours)
- Hunger/satiety and acceptance of the meals(Postprandial period of 5 hours (Time points: fasting (0) and 15, 30, 45, 60, 90, 120, 180, 240 and 300 minutes))
研究者
Prof. Dr. Sarah Egert
Sarah Egert, Prof PhD
University of Bonn