Characterization of Exosomes Platelets-released
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 175
- 试验地点
- 1
- 主要终点
- Characterization of exosomes released by activated platelet by Nanoparticle Tracking Analysis (NTA) and proteoma analysis
研究概览
简要总结
Extracellular vesicles (EVs) play a key role in cell-to-cell communication. They are small vesicles that contain rich molecular cargo. Recently, they have been proposed as biomarkers for clinical diagnostics. EVs include three classes: small EVs (exosomes), large EVs (microparticles), and apoptotic bodies. Platelet-derived EVs (PEVs) are the most abundant class in human blood and can actively participate in numerous physiological and pathological processes. The information about the role of platelet exosomes in cardiovascular disease and the effect of antiplatelet agents on their release and content is very limited.
详细描述
This study is a multicentre, observational. We plan to enrol 54 subjects from Centro Cardiologico Monzino IRCCS, and 121 buffy coat related to 121 subjects from Centro Trasfusionale Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico. biographical and anthropomorphic data will be recorded, a fasting blood venous sample (from ante-cubital vein) will be collected for the haematochemical analyses and for research samples.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Other
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •subjects without known pathologies
排除标准
- •individuals with gastrointestinal diseases,
- •individuals with history of hepatic diseases,
- •individuals with history of urogenital diseases,
- •individuals with history of hematological diseases,
- •individuals with history of immunological diseases,
- •individuals with history of renal diseases,
- •individuals with history of metabolic diseases
- •individuals with history of respiratory diseases
- •individuals with history of cancer
- •individuals with history of cardiovascular disease
- •individuals having permanent organ damage
- •individuals with major trauma in the last 6 months prior to enrollment
- •individuals with surgery in the last 6 months prior to enrollment
- •individuals taking medication in the 15 days prior to enrolment.
结局指标
主要结局
Characterization of exosomes released by activated platelet by Nanoparticle Tracking Analysis (NTA) and proteoma analysis
时间窗: 36 month
Characterization of exosomes released by activated platelet and exposed to platelet inhibitors by NTA and proteoma analysis
时间窗: 36 month
次要结局
- Platelet exosome profile emerged by NTA and proteoma analysis will be related with the "biographical" and "anthropometric" data of the subjects.(40 month)