Serum Amyloid A Protein And Fetuin A Protein Levels In Periodontal Health And Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 主要终点
- Serum Amyloid A (SAA) levels as an inflammatory marker in periodontal disease both in serum and GCF
研究概览
简要总结
The purpose of this study was to evaluate acute phase proteins (APPs) Fetuin-A and Serum Amyloid A (SAA) levels in gingival crevicular fluid (GCF) and serum samples in periodontal health and disease.
详细描述
The study population consisted totally 60 subjects consulted to Bulent Ecevit University, Faculty of Dentistry, Department of Periodontology whose age and sex were matched.
According to study design, subjects were divided into three groups based on their periodontal parameters. Patients suffering from chronic periodontitis formed the group 1 (n: 20), patients with plaque induced gingivitis formed the group 2 (n:20) and periodontal healthy patients were inserted in group 3 (n:20).
After gently drying the area, GCF was sampled with filter paper using the intracrevicular method (Periopaper, ProFlow, Inc., Amityville, New York, USA).Two milliliters (ml) of peripheral blood was collected from each individual with sterile syringe from the antecubital fossa by venipuncture, and to separate serum component sample centrifuged at 3000g for 5 min, and immediately stored at -40°C until assayed.
The quantity of Fetuin-A and SAA protein concentration in the samples were analyzed by sandwich enzyme-linked immunosorbent assay (ELISA) procedures using commercially available kits.
The Spearman's rank correlation test was used to detect the relationship between GCF and serum SAA and Fetuin-A with GI and CAL. All tests were performed using statistical software (SPSS Inc., version 22.0, Chicago, IL, USA). The mean ± standard deviation with mean rank values were calculated for each parameter, based on the patients as the statistical unit. P < 0.05 was considered to be statistically significant.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 28 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •GI>1, PPD≥5mm, CAL≥5mm with alveolar bone loss radiographically.
- •Systematically healthy patients
- •Group 2 Inclusion Criteria:
- •No alveolar bone and attachment loss
- •Inflammation signs such as redness, edema and increased BOP levels,
- •GI≥2, PPD≤3mm, CAL≤3mm.
- •Systematically healthy patients
- •Group 3 Inclusion Criteria:
- •No bone and attachment loss,
- •GI=0, PPD≤3mm, CAL≤3mm
- •Systematically healthy patients
- •Exclusion Criteria for all groups:
- •Aggressive Periodontitis,
- •Oral pathologies,
- •Patients with any other systemic diseases,
- •Pregnant women and those in the lactation period,
- •Patients with smoking habit and taking medication
- •Patients received periodontal therapy in last 6 months
排除标准
- 未提供
结局指标
主要结局
Serum Amyloid A (SAA) levels as an inflammatory marker in periodontal disease both in serum and GCF
时间窗: Day 0
次要结局
- Fetuin-A levels as an inflammatory marker in periodontal disease both in serum and GCF(Day 0)
研究者
ÇİĞDEM COŞKUN TÜRER
Dr.
Bulent Ecevit University