A Validation Study of Near Infrared Fluorescence Imaging of Lymphatic Vessels in Humans.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- refill time
研究概览
简要总结
The aim of this study is to examine the use of Near Infrared Fluorescence imaging on lymphatic vessels in humans.
Different parameters that reflect lymphatic function will be tested for intra and inter individual reproducibility.
We use 10 healthy test subjects and examine their lymphatic system. All subjects are healthy men between the ages of 20 and 30.
When the fluorescent dye "indocyanine green", has been injected into the skin on the patients feet, it will be transported through the lymph vessels, resulting in clear visualization of the vessels, using a laser and a EM-CCD camera.
On both legs we examine the frequency of vessel contraction and the velocity of lymph moving in vessels. We furthermore use several methods to quantify the vessel function such as the refill time of a vessel, (manually emptied vessel and refill time of 10 cm of a vessel), and pumping pressure (at what pressure lymph can pass a blood pressure cuff).
We investigate the activity of vessels over time, and after several interventions such as exercise, and water baths of the foot at different temperatures.
The patients are examined for approximately 4 hours and wil be examined again 14 days later.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- •lymphedema, edema or vascular disease.
结局指标
主要结局
refill time
时间窗: At baseline and after 14 days
Pumping pressure
时间窗: At baseline and after 14 days
Frequency of contraction in lymph vessels.
时间窗: At baseline and after 14 days
velocity
时间窗: At baseline and after 14 days
次要结局
未报告次要终点