Resveratrol-mediated Changes in Extracellular Vesicle microRNA Cargo as a Novel Contributor to Resistance Training-induced Skeletal Muscle Adaptations in Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 36
- 试验地点
- 2
- 主要终点
- Circulating extracellular vesicle microRNA profile
研究概览
简要总结
The main goal of this study is to find out if supplementing with resveratrol improves skeletal muscle adaptations to strengthening exercise (resistance training or strength training) in adults age 60+, and to determine whether circulating vehicles of inter-organ communication (extracellular vesicles) and their cargo might help explain those responses. The investigators will also examine whether resveratrol supplementation improves blood vessel function and cognitive function (brain health) in response to strength training.
详细描述
After screening and providing informed consent, participants (healthy older adults, age 60+, minimally active) will be familiarized with the procedures and undergo pre-testing. After pre-testing, participants will be assigned to supplement with resveratrol or placebo (double-blinded) daily while undergoing a strength training intervention. Strength training will be performed 3x/week for two 6-week blocks, and each visit will be supervised by a member of the study team. An additional week of testing will follow each of the two 6-week blocks.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 60 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Minimally active (less than 1 hour of structured exercise/week other than walking)
- •No resistance training for the previous 6 months
- •Not currently taking resveratrol supplements
- •Stable medication, supplement, dietary, and physical activity regimens for the previous 3 months and no plans to change throughout the study period
- •Body mass at least 110 lbs
排除标准
- •Medical complications that could increase risk during exercise (e.g., cardiovascular disease, uncontrolled hypertension, type 1 or 2 diabetes, orthopedic contraindication to exercise, kidney disease, etc.)
- •Cannabis or nicotine use
- •Consume more than 7 alcohol-containing beverages per week
研究组 & 干预措施
Resveratrol (RSV) group
Participants will be randomized to consume resveratrol (500 mg) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
干预措施: Resistance training (Behavioral)
Resveratrol (RSV) group
Participants will be randomized to consume resveratrol (500 mg) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
干预措施: Resveratrol 500 mg oral once daily. (Dietary Supplement)
Placebo
Participants will be randomized to consume placebo capsules (cellulose, visually identical to resveratrol capsules) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
干预措施: Resistance training (Behavioral)
Placebo
Participants will be randomized to consume placebo capsules (cellulose, visually identical to resveratrol capsules) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
干预措施: Placebo (Dietary Supplement)
结局指标
主要结局
Circulating extracellular vesicle microRNA profile
时间窗: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Circulating extracellular vesicles will be isolated from plasma and their microRNA cargo will be sequenced.
Muscle torque
时间窗: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Participants will be instructed to try and straighten their leg, against resistance, as forcefully as possible and maximal torque of the quadriceps assessed using a dynamometer.
Muscle fatigability
时间窗: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Participants will perform 32 repetitions of knee extensions on a dynamometer and the change in torque from the beginning to the end will be used to indicate fatigability.
Muscle strength
时间窗: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Participants will perform submaximal testing to assess strength on key exercises included in the strength training protocol.
Appendicular skeletal muscle mass index
时间窗: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Assessed using dual-energy x-ray absorptiometry (DEXA)
次要结局
- Waist-to-hip ratio(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Vascular function(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Cognitive function(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Grip strength(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Lower body strength/endurance(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Gait speed(During pre-, mid-, and post-testing (each separated by about 6 weeks))