Dietary Oxalate and Immune Cell Function
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 82
- 试验地点
- 2
- 主要终点
- Change in monocyte cellular energetics after 5 hours
研究概览
简要总结
The purpose of this study is to evaluate mitochondrial function in white blood cells and platelets from healthy individuals following dietary oxalate intake.
详细描述
Inflammation and dietary oxalate have been shown to play an important role in the development of kidney stones. Oxalate is a small molecule found in plants and plant-derived food. Individuals that have high oxalate intake have an increased risk of developing kidney stones. The co-investigators have previously shown that healthy subjects that consume dietary oxalate have increased plasma and urine oxalate.
White blood cells are essential for the immune response and rely on the mitochondria to carry out important cell functions. The Principal Investigator's current research shows that patients with calcium oxalate kidney stones have decreased mitochondrial function in their immune cells. Using the expertise of the investigators, this study will test whether oxalate has a direct effect on mitochondrial function in immune cells from healthy subjects. This information could help us understand the role of oxalate on the immune system during kidney stone pathogenesis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Mentally competent adults who are able to read and comprehend the consent form, written in English
- •Male or Female
- •Age 18 to 70 years
- •Non-tobacco users
- •BMI between 19 and 27
- •Willingness to abstain from vigorous exercise during study period
- •Normal blood comprehensive metabolic panel
排除标准
- •Mentally incompetent adults who are unable to read or comprehend the consent form, written in English
- •Pregnant females
- •Active medical problems
- •History of kidney stones
- •Any medical disorder that could influence absorption or excretion of oxalate
- •BMI less than 19 or greater than 27
- •Tobacco users
- •Current use of medications or dietary supplements
研究组 & 干预措施
3d Diet+Kale Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On the final day, participants will return to the study site and drink a prepared kale smoothie and be provided a breakfast meal.
干预措施: Kale smoothie (Dietary Supplement)
3d Diet+Blended Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended smoothie and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+V Spinach Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended spinach smoothie with varying amounts of spinach and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+Blended Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended smoothie and be provided a breakfast meal.
干预措施: Blended smoothie (Dietary Supplement)
3d Diet+V Spinach Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended spinach smoothie with varying amounts of spinach and be provided a breakfast meal.
干预措施: V spinach smoothie (Dietary Supplement)
3d Diet+Spinach Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to study site and drink a prepared blended spinach smoothie and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+Spinach Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to study site and drink a prepared blended spinach smoothie and be provided a breakfast meal.
干预措施: Spinach smoothie (Dietary Supplement)
5d Diet+Spinach Smoothie+Breakfast
5 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended spinach smoothie and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
5d Diet+Spinach Smoothie+Breakfast
5 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared blended spinach smoothie and be provided a breakfast meal.
干预措施: Spinach smoothie (Dietary Supplement)
3d Diet+Kale Smoothie+Breakfast
3 days of prepared meals with fasting on the final day. On the final day, participants will return to the study site and drink a prepared kale smoothie and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+Sodium Oxalate Drink+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared sodium oxalate drink and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+Sodium Oxalate Drink+Breakfast
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a prepared sodium oxalate drink and be provided a breakfast meal.
干预措施: Sodium oxalate drink (Dietary Supplement)
3d Diet+Spinach Smoothie+Breakfast w/ 24 Hr Urine
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a blended spinach smoothie and be provided a breakfast meal.
干预措施: Breakfast (Dietary Supplement)
3d Diet+Spinach Smoothie+Breakfast w/ 24 Hr Urine
3 days of prepared meals with fasting on the final day. On final day, participants will return to the study site and drink a blended spinach smoothie and be provided a breakfast meal.
干预措施: Spinach smoothie (Dietary Supplement)
结局指标
主要结局
Change in monocyte cellular energetics after 5 hours
时间窗: 0 hours - 5 hours
Cellular bioenergetics (oxygen consumption rate) will be measured in isolated monocytes from human blood using a Seahorse XF Analyzer machine. Monocyte cellular bioenergetics will be measured before and after consuming a spinach smoothie (pre- and post-loads; Time 0 hours and 5 hours) to assess a change in monocyte cellular energetics after 5 hours.
次要结局
未报告次要终点
研究者
Tanecia Mitchell, PhD
Principal Investigator
University of Alabama at Birmingham