To evaluate the effect of vibratory stimulation and low level laser therapy on the levels ofInterlukin-1 Beta, Interlukin-8 and amount of Tooth Movement

Purpose & Background: Orthodontic treatment takes a long time therefore different methods like lasers and vibrations separately were used to increase orthodontic tooth movement thereby reducing treatment time. However, some studies showed a significant increase in tooth movement and some did not. Therefore, the purpose of this study is to compare the orthodontic tooth movement with the use of vibrations and lasers together with only lasers and without lasers or vibrations. If there is an increase in tooth movement the total treatment time will be reduced and therefore will be beneficial to both patient and the clinician.

30 patients coming to the Department of Orthodontics and Dentofacial Orthopaedics, Inderprastha Dental College and Hospital  requiring maxillary first premolar extraction will be considered for the study.

v  After patient selection, the patient will be informed about the study and if he/she agrees with the protocol, the consent form will be signed by the patient.

v All patients will  receive repeated oral hygiene instructions.

v (T0) : After both maxillary first premolar extraction a sample of GCF  will be collected to estimate the levels of IL-1 Beta and IL-8 from the pressure site of the right maxillary canine. Enzyme Linked Immunoassay (ELISA) would be done for this evaluation.

v Patients would be divided into two groups  having 15 patients each.

v Levelling and aligning procedures will be done.

v (D1): Distance between the cusp tip of canine and mesio buccal cusp tip of 1st molar will be measured using calliper.

v This is followed by canine retraction, which will be done by NiTi closed coil spring positioned from the canine bracket to the first molar buccal tube.

v Same material and method will be applied for canine retraction for both the groups.

v GROUP 1: No additional stimuli will be given.

v GROUP 2 : Patients of this group will be given a mechanical tooth brush with a high frequency of 150 Hz and will be asked to brush with it twice a day for six minutes each time (12 minutes).

Ø Laser Irradiation: On the right side of the maxilla of the patients of group 2 laser irradiation will be carried out.

v A medium InGaAsP Semi-conductor diode laser equipment (BIOLASE EPIC LASER)  will be used for the study, emitting infra-red radiation at 940nm, at a continuous wave mode. All irradiations will be done by the same operator with an output power of 100mW, and exposure time of 3 second at each point.

The tip will be held perpendicular to and in contact with the gingival mucosa during the laser irradiation procedure. Protection glasses were worn by both the operator and the patients. Total of 10 irradiations will be carried out each time, five on the buccal side and five on the palatal side of the canine of the laser group:v Two mesial,

v One medial,

v Two distal.

v Laser irradiation will be done  on 1st, 7th, 15th, 30th, 45th, 60th, 75th , 90th day of canine retraction.

v The procedure will be followed on all subsequent appointments.

v (T1): GCF fluid samples will again be collected at the end of three months from both the groups, which will be analysed for the levels of IL-1 beta and IL-8.

v (D2): The distance between the cusp tips of the canine and the mesio buccal cusp tip of the first molar will be measured with a caliper.

v The collected data will be compared and statistically analysed.

v ANOVA test will be carried out.

v A statistician will be consulted for expert opinion and modifications will be made if necessary.