Clinical Significance of Circulating Non-coding RNA in Acute Ischemic Stroke (AISRNA)
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 500
- 试验地点
- 1
- 主要终点
- Differential expression pattern of circRNA/miRNA/lncRNA
研究概览
简要总结
AISRNA is to analyze the expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) by next-generation sequencing in patients with acute ischemic stroke and healthy control. The candidate circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke.
详细描述
Noncoding RNAs have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of this protocol will investigate the expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) by next-generation sequencing in patients with acute ischemic stroke and healthy control. The candidate circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke. Distinctive expression patterns of circRNA/miRNA/lncRNA will be identified by the next-generation sequencing and individual quantitative real time polymerase chain reaction (qRT-PCR). A diagnostic or predictive model will be established using logistic regression. The panel of these altered ncRNAs may be associated with acute IS and could serve as a novel diagnostic or predictive method.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Aged 18 years or older
- •Confirmed acute ischemic stroke by a diffusion-weighted imaging-position lesion on magnetic resonance imaging (MRI) and a new lesion on a brain computed tomography (CT) scan
- •Within 72 hours of symptom onset
- •Good performance status
- •Signed an approved informed consents
排除标准
- •a history of hemorrhagic infarction, chronic kidney/liver diseases, peripheral arterial occlusive disease, active malignant disease, and inflammatory or infectious diseases
研究组 & 干预措施
AIS group
This group includes patients with acute ischemic stroke (AIS).
干预措施: Sequencing of circRNA/miRNA/lncRNA (Genetic)
HC group
This group includes healthy controls (HC).
干预措施: Sequencing of circRNA/miRNA/lncRNA (Genetic)
结局指标
主要结局
Differential expression pattern of circRNA/miRNA/lncRNA
时间窗: 90 days
Differential expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) will be compared between AIS group and HC group, thus candidate circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of AIS.
次要结局
- Prognostic value of circRNA/miRNA/lncRNA in acute ischemic stroke(90 days)
- Correlation of circRNA/miRNA/lncRNA and inflammatory factors in acute ischemic stroke(7 days)
- Correlation of circRNA/miRNA/lncRNA and stroke-associated infection(30 days)
- Dynamic changes of circRNA/miRNA/lncRNA during the follow-up period(90 days)