Effects of a Short Term Caloric Restriction on T Cell Activation in Fat
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- Changes in T cell activation in adipose tissue in response to calorie restriction
研究概览
简要总结
Overweight and obesity are major problems and their complications such as cardiovascular disease and type 2 diabetes mellitus pose great burdens on healthcare systems. There is accumulating evidence to support obesity being a chronic inflammatory disorder mediated in part by the expansion of adipose (fat) tissue.
In addition to adipocytes (fat cells), adipose tissue contains a whole range of other cell types including some immune (white blood) cells. The relative proportions of immune cell subpopulations and interactions between the different cell types within adipose tissue may be important in the development of type 2 diabetes.
Previous research by the investigators has suggested that there may be important differences in the activation status of certain immune cells located in adipose tissue with increased overweight and the production of leptin - an appetite regulating hormone. Properties of adipose tissue and inflammatory cells located within it may therefore be sensitive to changes in our nutritional status.
The purpose of the present study is to investigate changes in T cell activation in adipose tissue alongside other immunometabolic properties before and after a short period of dieting compared to changes in blood.
Participants will include males aged between 35-55 years old who meet the criteria for inclusion. After taking some preliminary measurements and monitoring of normal daily activities, participants will reduce their calorie intake to 50 % of their normal intake/requirements for 3 consecutive days and will attend 1 session of Laboratory testing before and 1 after this period which will take place in the Physiology Laboratories at the University of Bath.
It is hoped that investigating immunometabolic responses within adipose tissue following an acute nutritional challenge will help lead to a better understanding of the development of diseases associated with being overweight and ultimately help in the development of more effective methods for prevention and treatment.
详细描述
The aim of this research is to investigate whether the immunometabolic properties of adipose tissue and the inflammatory cells located within it can be modified by short term changes in nutritional status.
Following advertisement of the study, interested potential participants will be asked to contact the Chief Investigator for further information via email/telephone correspondence. There will be an initial assessment of eligibility based on inclusion/exclusion criteria and, if these requirements are met, the potential participant will be invited to a meeting to further discuss the trial. After they have read the participant information sheet and seen the flowchart outlining the timeline for the study, if they would like to take part, they will be asked to sign a consent form. Dates will then be scheduled for some preliminary anthropometric measurements, 1 week of diet and physical activity monitoring and 3 days of calorie restriction with trial dates either side.
Preliminary measurements:
Preliminary testing will include anthropometric measurements of height, weight, waist and hip circumferences, sagittal waist height and blood pressure. Participants will also have their body composition precisely assessed using a dual energy X-ray absorptiometer (DEXA). These measurements will take place at the University of Bath Physiology Laboratories.
Monitoring of energy balance:
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 35 Years 至 55 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Waist circumference > 94 cm
- •Weight stable for more than 3 months (no change in weight +/- 3 %)
- •Non-smoker
排除标准
- •Personal history of/existing cardiovascular disease, metabolic disease or dyslipidaemia
- •Taking medications that may influence lipid or carbohydrate metabolism or immune system function
结局指标
主要结局
Changes in T cell activation in adipose tissue in response to calorie restriction
时间窗: 3 days
T-lymphocytes will be isolated from adipose tissue samples taken before and after 3 days of 50 % calorie restriction and levels of their activation will be assessed by flow cytometry.
次要结局
- Changes in T cell activation in blood in response to calorie restriction(3 days)
- Changes in IL-6 mRNA expression by adipose tissue(3 days)
- Changes in IL-6 secretion by adipose tissue(3 days)
研究者
Rebecca Travers
Principal Investigator
University of Bath