Comparative evaluation of Photodynamic therapy with conventional scaling and root planing at different frequencies in chronic periodontitis patient: A Clinico-microbiological study

This study is a randomized, double blinded, single center trial comparing and evaluating the clinical and microbial effects of photodynamic therapy using low level laser of 660nm wavelength and 0.1 mg/ml of Toluidine blue O dye irrigated for 60 seconds. Low level laser exposure done for 1 minute using swiping motion from apical to coronal direction within the periodontal pocket on the assigned treatment side. Each quadrant will be receiving scaling and root planing. one of the quadrant will receive one time exposure of Photodynamic therapy, other quadrant will be receiving two time exposure of Photodynamic therapy, In the remaining two quadrant one side will be receiving scaling and root planning treatment along with irrigation of 0.1 mg/ml of toluidine blue O dye while the other will be receiving scaling and root planing. Primary outcome is by assessing the clinical parameters such as gingival index, bleeding index, plaque index, periodontal probing depth and clinical attachment level which will be recorded during each appointments. Secondary outcome is by assessing the microbiological analysis by counting the colony forming units based on the samples drawn after each appointments.