Effect of Fructose and Glucose and TAS1R2 in Glucose, Triglycerides, Uremia, Oxidative Stress, Feelings Related to Food Intake of Individuals With Type 1 Diabetes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 16
- 试验地点
- 2
- 主要终点
- Compare the effects of fructose and glucose and TAS1R2 in postprandial metabolism of individuals with type 1 diabetes
研究概览
简要总结
- BACKGROUND: Individuals with diabetes report innate preference for sweet foods, possibly due to genetic variants. In addition, studies have shown that ingestion of fructose promotes lower postprandial blood glucose, compared to glucose. However, excessive intake may increase triglycerides, uric acid and oxidative stress, due to oxidative priority.
- AIMS: To investigate the influence of fructose and glucose and taste receptor, type 1, member 2 (TAS1R2) in glucose, triglycerides, uremia, oxidative stress, feelings related to food intake and palatability of individuals with type 1 diabetes.
- METHODS: The trial is a single-blind, two-way crossover (1-week washout) study in 30 subjects with type 1 diabetes. Blood samples were collected before and 2-hours after the participants receive 75g of fructose or 75g of glucose dissolved in 200 ml water. Capillary blood glucose were assessed at 30, 90, 120, and 180 minutes to determine glucose, and visual analogue scales for measurement of appetite sensation were assessed at 70, 120, and 190 minutes.
- PURPOSE: The research proposal adds knowledge about the TAS1R2 (Ile191Val) polymorphism and around the most suitable monosaccharide for individuals with diabetes type 1.
详细描述
INTRODUCTION:
Fructose is transported via the portal vein to the liver, where is rapidly taken up by hepatocytes and phosphorylated to fructose-1-phosphate independent of insulin action by the enzyme fructokinase. Thus, under normal conditions, fructose is converted mainly to glucose, glycogen, and lactate, and to a small extent also to triglycerides. However a hypertriglyceridemia has been occur during high intakes of fructose.
Large amounts of fructose, unlike glucose, may result in increased lactate, pyruvate, and uric acid level in healthy subjects and patients with uncontrolled diabetes. However, uric acid also has pro-oxidative properties. Possibly, the uric acid neutralizes oxidative damage.
Sweetness is very attractive to patients with diabetes possibly due to genetic variants. The sweet taste receptor is a heterodimer of 2 protein subunits, T1R2 (taste receptor, type 1, member 2) and taste receptor, type 1, member 3, located on human chromosome 1. T1R2 is the component specific to sweet taste perception because taste receptor, type 1, member 3 is also involved in the detection of umami when it dimerizes with taste receptor, type 1, member 1.
At moment, none study assessed TAS1R variants in individuals with type 1 diabetes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Health Services Research
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Type 1 diabetes ( ≥ three years after diagnosis)
- •Normal-weight (BMI between 18.5 e 25.0 kg/m2)
- •Using basal-bolus regimen with insulin infusion pump or insulin analogs (basal: detemir or glargine; bolus: lispro, glulisine or aspart).
排除标准
- •Drinkers;
- •Use of exogenous hormone (with the exception of insulin);
- •Use of antibiotics, anti-inflammatories or oral antidiabetics pharmacotherapy;
- •Diagnosis of nephropathy;
- •Diagnosis of liver diseases;
- •With other types of diabetes family history;
- •Visual disturbances.
结局指标
主要结局
Compare the effects of fructose and glucose and TAS1R2 in postprandial metabolism of individuals with type 1 diabetes
时间窗: baseline up to 3 hours each day study. Total study: 2 years
Determine the blood glucose, triglycerides, oxidation of substrates, uremia, oxidative stress, feelings related to food intake of individuals with type 1 diabetes, before and 3-hours after ingestion of 75g of fructose or 75g of glucose.
次要结局
- Effect of two polymorphisms in the gene TAS1R2 in the sweet taste perception(only at baseline)
- Effect of fructose or glucose on glucagon levels(Baseline, and up to 3-hours after solutions)
- Effect of fructose or glucose on appetite and palatability(Baseline, and up 120 minutes)
- Effect of fructose or glucose on uric acid, pyruvic acid, and lactate(Baseline, and up to 3-hours after solutions)
- Effect of fructose or glucose on oxidative stress(Baseline, and up to 3-hours after solutions)
- Effect of fructose or glucose on triglycerides levels(Baseline, and up to 3-hours after solutions)
- Effect of fructose or glucose on leptin levels(Baseline, and up to 3-hours after solutions)
- Effect of fructose or glucose on blood glucose(Baseline, and up to 3-hours after solutions)
研究者
Débora Lopes Souto
Ph. D. student in Nutrition Sciences at the Federal University of Rio de Janeiro
Universidade Federal do Rio de Janeiro