Influence of Lifestyle Factors, Cardiorespiratory Fitness, and Body Composition on Plasma BDNF and pTau217 Levels and Cognitive Function in Healthy Young Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 31
- 试验地点
- 1
- 主要终点
- Plasma biomarkers
研究概览
简要总结
Lifestyle play a key role in modulating brain health by directly influencing a number of key biological processes, such as neuroplasticity, neurogenesis, and regulation of biomarkers associated with cognitive decline. Factors such as physical activity, diet, sleep quality, stress management, and body composition have been shown to have a significant impact on neuroprotection and cognitive performance. These factors not only contribute to maintaining metabolic and functional balance in the body, but are also linked to the expression of biomarkers such as brain-derived neurotrophic factor (BDNF) and Tau protein phosphorylated at the amino acid threonine 217 (PTau217), which are essential for the maintenance of neuronal health and the prevention of neurodegenerative diseases. In this sense, a healthy lifestyle, characterized by regular physical activity, a balanced diet, good quality sleep, low stress levels and adequate body composition, can act as a protective factor against cognitive decline from early stages of life.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 24 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •The following inclusion criteria are established:
- •Age between 18 and 24 years.
- •Not have chronic or neurodegenerative diseases.
- •Not be on pharmacological treatment that affects the central nervous system or metabolism.
排除标准
- •for participants:
- •Medical or traumatological condition that makes it impossible to practice physical exercise.
- •Musculoskeletal injury within 6 months prior to the first visit to the laboratory.
- •Recent surgery (<3 months).
- •Have a medical condition in which physical activity is contraindicated (assessed with the Physical Activity Predisposition Questionnaire (PARQ+).
研究组 & 干预措施
Healthy young adults
Healthy young adults (18-24 years old)
结局指标
主要结局
Plasma biomarkers
时间窗: Baseline
Plasma concentrations of BDNF and pTau217 will be assessed using ELISA kits. Fasting venous blood samples will be collected by a qualified nurse from the antecubital vein into EDTA-treated vacutainers and centrifuged at 1500g for 10 minutes. Plasma will then undergo a second centrifugation at 2500g for 15 minutes and be stored in cryogenic vials at -80ºC. BDNF will be measured using the Human/Rat BDNF ELISA Kit (Proteintech Europe; sensitivity: 0.1 pg/mL; range: 12.5-800 pg/mL). PTau217 will be measured using the Human Phosphorylated Tau 217 ELISA Kit (GenoChem World; sensitivity: 0.1 pg/mL; range: 0.8-40 pg/mL).
次要结局
- Physical activity levels(During one week prior to baseline)
- Sleep quality and cycle (objective)(During one week prior to baseline)
- Waist-hip ratio(Baseline)
- Hip circumference(Baseline)
- Waist circumference(Baseline)
- Sleep quality (subjective)(During one week prior to baseline)
- Heart rate variability(During one week prior to baseline)
- Stress levels(Baseline)
- Plasma testosterone/cortisol ratio(Baseline)
- Nutritional habits(Baseline)
- Handgrip strength(Baseline)
- Gas exchange analysis(Baseline)
- Cardiovascular response(Baseline)
- Excess Post-exercise Oxygen Consumption(Baseline)
- Weight(Baseline)
- Height(Baseline)
- Body surface area(Baseline)
- Bioimpedance(Baseline)
- Cognitive performance(Baseline)