The Effects of Animal- and Microbial-based Protein Sources on Whole-body Protein Metabolism in Healthy Adults
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- Daniel Moore
- 入组人数
- 13
- 试验地点
- 2
- 主要终点
- 1. Phenylalanine excretion (umol/kg/h)
研究概览
简要总结
Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy, male and female, recreationally-active participants.
- •Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
- •Participants will be aged 18-45 years old.
- •Participants are willing to abide by the compliance rules of this study.
- •Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
- •Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).
排除标准
- •Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
- •Self-reported regular tobacco use.
- •Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
- •Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- •Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
- •Use of COCs containing >21 active pills per cycle due to potential differences in their effect on metabolism (female participants).
- •Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants).
- •Not currently or previously (in the past 6 months) on a vegan diet
结局指标
主要结局
1. Phenylalanine excretion (umol/kg/h)
时间窗: 8 hours
Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).
Phenylalanine oxidation (umol/kg/h)
时间窗: 8 hours
Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.
次要结局
未报告次要终点
研究者
Daniel Moore
Principal Investigator
University of Toronto