Examining the Impact of Acute Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males: A Randomized Cross-over Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 13
- 试验地点
- 2
- 主要终点
- Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)
研究概览
简要总结
This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Investigator, Outcomes Assessor)
盲法说明
Allocation concealment, assessor blinding
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •3 hours of physical activity per week
- •No previous cycling training
- •No concurrent involvement in another training program
- •Body mass index < 30 kg/m2
排除标准
- •Presence of cardiovascular disease
- •Presence of metabolic disease
- •Taking regular oral medication
- •Current smoker
研究组 & 干预措施
CTL - BFR
CTL Exercise then BFR Exercise
干预措施: BFR Exercise (Other)
CTL - BFR
CTL Exercise then BFR Exercise
干预措施: CTL Exercise (Other)
BFR - CTL
BFR Exercise then CTL Exercise
干预措施: BFR Exercise (Other)
BFR - CTL
BFR Exercise then CTL Exercise
干预措施: CTL Exercise (Other)
结局指标
主要结局
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)
时间窗: 15 minutes before exercise and 3 hours post-exercise for both exercise sessions
Fold-change in PGC-1α mRNA expression
次要结局
- Catecholamine concentrations(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Total and phosphorylated acetyl coenzyme A carboxylase (ACC)(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Total and phosphorylated protein kinase A (PKA)(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Heart rate (HR)(During both exercise sessions)
- Total and phosphorylated p38 mitogen-activated protein kinase (MAPK)(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Blood lactate(During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions)
- Oxygenated and deoxygenated hemoglobin(During both exercise sessions and up to 10 minutes after (for cuff occlusion))
- Acetylated p53(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Revolutions per minute (RPM)(During both exercise sessions)
- Ratings of perceived exertion (RPE)(During both exercise sessions)
研究者
Brendon Gurd
Associate Professor
Queen's University