The Effect of β-hydroxy-β-methylbutyrate Supplementation on Muscle Damage Induced by Resistance Exercise
试验速览
- 阶段
- 2 期
- 发起方
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Changes in the maximum voluntary isometric contraction (MVIC) after supplementation and exercises-induced muscle damage
研究概览
简要总结
The study aims at assessing the influence of β-hydroxy-β-methylbutyrate (HMB) supplementation (4.0 g/day) supplemented for 7 days in a group of speed-strength trained individuals on lower-body strength performance, selected muscle histological, molecular and blood markers of muscle recovery in response to resistance exercise-induced muscle damage.
详细描述
The main aim of the project is to assess the effects of seven days of HMB (4.0 g/day) or Placebo (PLA) supplementation on lower body strength performance, and functional, histological, clinical and molecular markers of muscle recovery after an acute resistance exercise session.
The study is a double-blind, randomized, placebo-controlled, parallel trial. 30 male participants will be divided randomly into two parallel groups: One group receiving 4.0 g/day HMB and the second group receiving PLA. Supplementation period (HMB or PLA) will be seven days (five days before and two days after muscle damage induced by an acute resistance training session). Food intake during the intervention will be assessed with the use of a food diary.
Before the start of the experimental protocol, the participants will be familiarized with all tests and procedures.
During the first visit, participants will be assessed for their anthropometric characteristics, and baseline blood samples will be collected for the analysis of the activity of muscle damage markers [creatine kinase (CK) and lactate dehydrogenase (LDH)]. We will then carry out the evaluation of the maximal voluntary isometric contraction (MVIC; performed in a Biodex isokinetic dynamometer), lower body maximum dynamic strength (1RM) for squat, leg press, and leg extension exercises. The participants will then return to the laboratory to repeat the same tests and confirm the obtained results (MVIC and 1RM tests).
On the third day, participants will take part in a resistance training session (RE3sets) to evaluate strength performance. We will monitor the maximum number of repetitions possible in three sets of three exercises (squats, the leg press, and leg extension), until the concentric failure, with a rest interval of two minutes per set. The workload will correspond to 80% of the individual's 1RM).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •written informed consent from all participants before the study
- •a current medical clearance to practice sports,
- •training experience: at least 1 year strength training programs that include lower limbs,
- •minimum of 3 workout sessions (strength/resistance training) a week,
- •protein intake in customary diet and during the study protocol > 1.6g / kg of body mass per day,
- •individual maximum strength greater than 2.5 times the body weight for the 1RM test in the leg-press.
排除标准
- •current injury,
- •any health-related contraindication,
- •declared general feeling of being unwell,
- •unwilling to follow the study protocol,
- •serious disease or metabolic problems,
- •intake of creatine, caffeine or beta-alanine, or other ergogenic supplements 3 months before the beginning of the study,
- •history of anabolic androgenic steroids or drugs use that may interfere with muscle mass control (eg, corticosteroids), or affect physical performance,
- •smoking and tobacco use,
- •presence of infectious disease in the previous 4 weeks of the study.
结局指标
主要结局
Changes in the maximum voluntary isometric contraction (MVIC) after supplementation and exercises-induced muscle damage
时间窗: Baseline, 24 hours and 48 hours after the end of exercises-induced muscle damage
Maximum voluntary isometric contraction (MVIC) torque (Nm)
次要结局
- Change in the muscle cholesterol and mevalonate content (in the vastus lateralis muscle samples) after supplementation and exercises-induced muscle damage(Baseline, 24 hours and 48 hours after the end of exercises-induced muscle damage)
- Changes in creatine kinase (CK) and lactate dehydrogenase (LDH) activity (IU) in blood after supplementation and exercises-induced muscle damage(Baseline, 24 hours and 48 hours after the end of exercises-induced muscle damage)
- Change in the strength performance results (at 80% of the individual's 1RM) in three resistance exercises (squats, the leg press, and leg extension) before and after supplementation and exercises-induced muscle damage(Baseline and 48 hours after the end of exercises-induced muscle damage)
- Change in the histological sarcomere integrity (after biopsy of the vastus lateralis muscle samples) after supplementation and exercises-induced muscle damage(Baseline, 24 hours and 48 hours after the end of exercises-induced muscle damage)
- Change in the molecular potential (after biopsy of the vastus lateralis muscle samples) after supplementation and exercises-induced muscle damage(Baseline, 24 hours and 48 hours after the end of exercises-induced muscle damage)
研究者
Krzysztof Durkalec-Michalski
Prof Dr hab., PhD
Poznan University of Physical Education