The Differences of Oocyte Maturation, Granulosa Cell Apoptosis Index Between Electroacupuncture And Sham Groups IVF
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Index apoptotic (Ratio Bax/Bcl-2) of granulosa cells, GDF9 and BMP15
研究概览
简要总结
Interventional studies compared electroacupuncture group versus sham electroacupuncture group to granulosa cell apoptosis index, oocyte maturation, GDF9 and BMP15 levels, and fertilization rate in in vitro fertilization program
详细描述
The study was conducted at the Dr.Cipto Mangunkusumo National General Hospital, Indonesia in September - November 2018.The inclusion criteria are patients who underwent controlled ovarian stimulation with antagonist protocol, age 30-39 years, BMI <29kg / m2. Exclusion criteria were not having a skin infection at acupuncture point and not using a pacemaker. Then patients were randomly divided into two groups: electroacupuncture and sham electroacupuncture. Electroacupuncture therapy was done 6 times at: 2 times before and 4 times during controlled ovarian stimulation.Apoptotic index of granulosa cells were examined by quantitative real time PCR techniques for BAX/BCL-2 expression.Oocyte maturation and fertilization rate output parameters are examined by competent embryologists after ovum pick up while GDF9 and BMP15 levels were examined by quantitative real time PCR techniques for GDF9 and BMP15 mRNA expression
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 30 Years 至 39 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •women underwent controlled ovarian stimulation with antagonist protocol
- •BMI < 29 kg/m2
- •willing and sign the inform consent
- •follow the research until completed
排除标准
- •having skin infection at acupoint location
- •use a pacemaker
研究组 & 干预措施
Electroacupuncture
Electroacupuncture therapy is done at the point of CV3 Zhongji, CV4 Guanyuan, and EXCA-1 Zigong with continuous wave, 2 Hz frequency for 30 minutes. Acupuncture manuals on MA-IC3 endocrine (ear points), GV20 Baihui, ST36 Zusanli, SP6 Sanyinjiao, BL57 Chengsan and KI3 Taixi for 30 minutes.
干预措施: Electroacupuncture (Procedure)
Sham electroacupuncture
Sham electroacupuncture are done at same electroacupuncture point location but puncture are not done, also electro stimulator are turned off.
干预措施: Electroacupuncture (Procedure)
结局指标
主要结局
Index apoptotic (Ratio Bax/Bcl-2) of granulosa cells, GDF9 and BMP15
时间窗: "up to 1 months"
Bax/Bcl-2 is proapoptotic protein compare to antiapoptotic protein. GDF9 and BMP15 protein expression
次要结局
- oocyte maturation index("up to 1 months")
- Fertilization rate("up to 1 months")
研究者
nadia oktari
principal investigator
Indonesia University