The Role of Adipose Tissue in Adaptive Responses to Exercise
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- Mayo Clinic
- 入组人数
- 56
- 试验地点
- 1
- 主要终点
- Adipose tissue gene expression of inducible nitric oxide synthase (INOS) measured by PCR.
研究概览
简要总结
The purpose of this research is to determine how exercise affects fat (adipose) tissue and how changes to adipose tissue that occur during and after exercise might improve health in aging and obesity.
详细描述
Adipose tissue has important endocrine functions that influence metabolic health. Early evidence shows that adipose adapts to physiological stress, including exercise. The objective here is to determine how exercise-induced alterations in adipose tissue cellular composition and endocrine signaling may contribute to the beneficial adaptations to exercise in aging and obesity.
Immune cell populations and inflammatory signatures will be assessed in subcutaneous abdominal adipose tissue biopsies collected from obese and normal weight young and older adults before, immediately after, and 3 hours after a 30-min bout of cycling exercise at 70% of maximal oxygen consumption (VO2max). Mass spectrometry, Olink targeted inflammation assays, and RNA sequencing will be used for full proteomic and transcriptomic characterization of the adipose tissue secretome (the proteins and molecules secreted from the adipose tissue) and the cargo of extracellular vesicles isolated from plasma and media collected from cultured human adipose tissue explants generated from each time point.
Overall, the primary hypothesis of the proposed work is that a single bout of exercise triggers transient changes in adipose tissue paracrine/endocrine signals and immune cellular composition. The investigators propose that these responses contribute to the beneficial effects of exercise locally and in distal tissues and that the cumulative effects of acute changes in adipose tissue likely contribute to the positive alterations in adipose tissue associated with exercise training.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Adults between the ages of 18-35 OR 65-
- •BMI 18.5 kg/m2 - 40 kg/m2
- •Weight stable (≥ 3 months)
- •Generally healthy
排除标准
- •Participation in ≥ 30 minutes of structured physical activity ≥ 2 days per week.
- •Smoking/tobacco use.
- •Alcohol/substance abuse.
- •Pregnancy and breastfeeding.
- •Abnormal renal function.
- •Blood clotting disorders.
- •Coronary artery disease.
- •Uncontrolled thyroid disease.
- •Liver disease.
- •Use of medications known to influence the main outcomes of the study.
- •Orthopedic problems that may be aggravated by exercise.
- •Chronic disease at the discretion of the investigators.
研究组 & 干预措施
Older adults with obesity
Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Adipose Tissue Biopsy (Procedure)
Older adults with obesity
Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Exercise (Behavioral)
Older adults without obesity
Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Adipose Tissue Biopsy (Procedure)
Older adults without obesity
Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Exercise (Behavioral)
Young adults with obesity
Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Adipose Tissue Biopsy (Procedure)
Young adults with obesity
Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Exercise (Behavioral)
Young adults without obesity
Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Adipose Tissue Biopsy (Procedure)
Young adults without obesity
Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
干预措施: Exercise (Behavioral)
结局指标
主要结局
Adipose tissue gene expression of inducible nitric oxide synthase (INOS) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the pro-inflammatory macrophage marker INOS will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of monocyte chemoattractant protein-1 (MCP1) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine MCP1 will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of tumor necrosis factor alpha (TNFa) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine TNFa will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of leptin (LEPD) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the pro-inflammatory adipokine LEPD will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of adiponectin (ADIPOQ) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the anti-inflammatory adipokine ADIPOQ will be assessed in adipose tissue samples using RT-PCR.
Inflammatory proteins found in adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
The concentrations of 96 different inflammatory proteins (including various cytokines and adipokines) will be measured in adipose tissue lysates using the targeted inflammation Olink proximity extension assay panel. This assay measures all 96 proteins in a single assay and all are reported as normalized protein expression values.
Protein cargo of adipose extracellular vesicles measured using mass spectrometry
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed. From this media, extracellular vesicles will be isolated. In these extracellular vesicles, mass spectrometry will be performed to examine the entire proteome (all proteins) contained within these extracellular vesicles. Using the large number of proteins identified, the investigators will examine changes in categories of protein types (e.g., inflammatory proteins, metabolic proteins, transcriptional proteins).
Adipose tissue macrophage populations
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Numbers of macrophages in the adipose tissue will be assessed by immunohistochemistry.
Adipose tissue gene expression of interleukin (IL)-6 measured by polymerase chain reaction (PCR).
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine IL-6 will be measured in adipose tissue samples using real time (RT)-PCR.
Adipose tissue gene expression of interleukin (IL)-8 measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine IL-8 will be assessed in adipose tissue samples using real-time (RT)-PCR.
Adipose tissue gene expression of cluster of differentiation 68 (CD68) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the macrophage marker CD68 be assessed in adipose tissue samples using real time (RT)-PCR.
Adipose tissue gene expression of cluster of differentiation 163 (CD163) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the anti-inflammatory macrophage marker CD163 will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of cluster of differentiation 206 (CD206) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the anti-inflammatory macrophage marker CD206 will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of adhesion G-protein coupled receptor E (ADGRE) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the macrophage marker ADGRE will be assessed in adipose tissue samples using RT-PCR.
Adipose lymphocyte populations
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
The numbers of lymphocytes in the adipose tissue will be assessed by flow cytometry.
Adipose tissue gene expression of leptin (LEPD) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the pro-inflammatory adipokine LEPD will be assessed in adipose tissue samples using RT-PCR.
IL6 secreted from adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
The concentration of 96 IL6 will be measured using the targeted inflammation Olink proximity extension assay panel in media obtained from cultured adipose tissue explants. Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed for the assessment of secreted inflammatory proteins. T
Adipose tissue macrophage populations
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Numbers of macrophages in the adipose tissue will be assessed by immunohistochemistry.
Adipose tissue gene expression of interleukin (IL)-6 measured by polymerase chain reaction (PCR).
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine IL-6 will be measured in adipose tissue samples using real time (RT)-PCR.
Adipose tissue gene expression of interleukin (IL)-8 measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine IL-8 will be assessed in adipose tissue samples using real-time (RT)-PCR.
Adipose tissue gene expression of cluster of differentiation 68 (CD68) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the macrophage marker CD68 be assessed in adipose tissue samples using real time (RT)-PCR.
Adipose tissue gene expression of cluster of differentiation 163 (CD163) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the anti-inflammatory macrophage marker CD163 will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of cluster of differentiation 206 (CD206) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the anti-inflammatory macrophage marker CD206 will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of adhesion G-protein coupled receptor E (ADGRE) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the macrophage marker ADGRE will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of inducible nitric oxide synthase (INOS) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the pro-inflammatory macrophage marker INOS will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of monocyte chemoattractant protein-1 (MCP1) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine MCP1 will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of tumor necrosis factor alpha (TNFa) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the inflammatory cytokine TNFa will be assessed in adipose tissue samples using RT-PCR.
Adipose tissue gene expression of adiponectin (ADIPOQ) measured by PCR.
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Gene expression of the anti-inflammatory adipokine ADIPOQ will be assessed in adipose tissue samples using RT-PCR.
Inflammatory proteins found in adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
The concentrations of 96 different inflammatory proteins (including various cytokines and adipokines) will be measured in adipose tissue lysates using the targeted inflammation Olink proximity extension assay panel. This assay measures all 96 proteins in a single assay and all are reported as normalized protein expression values.
Protein cargo of adipose extracellular vesicles measured using mass spectrometry
时间窗: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed. From this media, extracellular vesicles will be isolated. In these extracellular vesicles, mass spectrometry will be performed to examine the entire proteome (all proteins) contained within these extracellular vesicles. Using the large number of proteins identified, the investigators will examine changes in categories of protein types (e.g., inflammatory proteins, metabolic proteins, transcriptional proteins).
次要结局
- Adipocyte cell size(Pre-exercise, immediately post-exercise, and 3 hours after exercise)
- Protein cargo of plasma extracellular vesicles measured using mass spectrometry extracellular vesicles(Pre-exercise, immediately post-exercise, and 3 hours after exercise)
- Adipocyte cell size(Pre-exercise, immediately post-exercise, and 3 hours after exercise)
- Plasma IL6 concentrations measured using the Olink Target Inflammation Panel(Pre-exercise, immediately post-exercise, and 3 hours after exercise)
- Protein cargo of plasma extracellular vesicles measured using mass spectrometry extracellular vesicles(Pre-exercise, immediately post-exercise, and 3 hours after exercise)
研究者
Hawley E. Kunz
Principal Investigator
Mayo Clinic